Radioimmunoassay Method for the Determination of 22-Oxacalcitriol, a Novel Analog of 1α, 25-Dihydroxyvitamin D_3 Having Valuable Clinical Potency, in Rat and Human Plasma

概要

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22-Oxacalcitriol is a synthetic analog of 1α, 25-dihydroxyvitamin D_3,which is expected to be a novel agent for the treatment of patients with secondary hyperparathyroidism, psoriasis, and/or breast cancer. A sensitive and practical RIA for 22-oxacalcitriol in rat and human plasma has been developed. A rabbit antiserum raised against 22-oxacalcitriol 3-hemiglutarate conjugated with bovine serum albumin was used in combination with a tritium-labeled 22-oxacalcitriol having high specific radioactivity. A plasma sample was extracted with ethyl ether and the extract was successively purified with Bond Elut^[○!■] C18 and a normal-phase HPLC prior to the RIA to remove interfering substances. Accuracy of the RIA was assessed by various studies including serial dilution and recovery tests. Intra- and inter-assay coefficients of variation were lower than 10%, and the quantification limit was low enough for practical use (33 pg/ml plasma). The present RIA will be useful for the pharmacokinetic studies of 22-oxacalcitriol in both preclinical and clinical stages.
社団法人日本薬学会の論文
1995-03-15