Studies on the Absorption, Distribution, Excretion and Metabolism of Ginseng Saponins. I. Quantitative Analysis of Ginsenoside Rg_1 in Rats

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Quantitative analysis of ginsenoside Rg_1,isolated from red ginseng (Panax ginseng C. A. MEYER) and one of the main saponins, was experimented in rats for the studies of the absorption, distribution, excretion and metabolism of ginseng saponins. The analysis procedure was developed as follows. Ginsenoside Rg_1 added to biological samples of rats was adsorbed on a Servachrom XAD-2 resin column after deproteinization with methanol. The adsorbed ginsenoside Rg_1 was eluted with 60% methanol aqueous solution and further subjected to thin-layer chromatography with chloroform-methanol-water (65 : 35 : 10,lower phase) as the developing solvent and 8% vanillin methanol solution/72% H_2SO_4 (1 : 5) as the detecting reagent. As reasonable recoveries and standard deviations were found in this procedure, the concentrations of ginsenoside Rg_1 in samples from rats treated with ginsenoside Rg_1 (100 mg/kg, p. o.) were determined by applying the described method.
公益社団法人日本薬学会の論文
1982-06-25