Analytical Studies on 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1,2,4-triazole Hydrochloride Dihydrate. II. : A Fluorometric Method Applicable to Animal Feed

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A fluorometric method for the determination o 1-(2-o-chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1,2,4-triazole hydrochloride dihydrate (1) was established. This highly sensitive method was applied to the analysis of animal feed spiked with the drug. Compound 1 was easily transformed into the fluorophore 4-amino-7-chloro-5-(o-chlorophenyl)-[1,2,4]triazolo[1,5-a]quinoline-2-carboxylic acid (2) in alkaline solution. After the extraction of 1 from the feed, 1 was converted into 2. The strong fluorescence of 2 allowed the establishment of a method with a quantitation limit of 5 mg/kg.Compound 1 in feed samples spiked at 10-500mg/kg was determined with a coefficient of variation of less than 10%. The concentration of 1,its uniformity in the whole feed and its stability can be evaluated by this method.
公益社団法人日本薬学会の論文
1986-07-25