A New Fluorometric Method for Latamoxef in Biological Materials Using 2,6-Diamino-3-nitrosopyridine
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概要
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A novel fluorometric assay method was established for latamoxef (LMOX), a new type of antibiotic. LMOX was converted into 1,2,4-dihydroxy-3-[(1-methyltetrazol-5-ylthio) methyl] but-2-enoic acid γ-lactone, in an acid solution. The lactone 1 reacted with 2,6-diamino-3-nitrosopyridine to give the fluorophore, 3-(5-amino-3H-imidazo [4,5-b] pyridin-2-yl)-2,4-dihydroxybut-2-enoic acid γ-lactone. The fluorescence was measured at 460 nm with excitation at 392 nm. In plasma and urine, 1-15 μg/ml and 20-300 μg/ml of LMOX were determined, respectively, with good precision through a simple technique. The sample volume was 100 μl. The reaction mechanism of the fluorescence reaction is discussed.
- 公益社団法人日本薬学会の論文
- 1985-02-25
著者
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北川 隆康
Shionogi Research Laboratories, Shionogi & Co., Ltd.,
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北川 隆康
Shionogi Research Laboratories Shionogi & Co. Ltd.
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平井 瑛三
Shionogi Research Laboratory, Shionogi and Co., Ltd.
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松浦 昭男
Shionogi Research Laboratories Shionogi And Co. Ltd.
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北川 隆康
塩野義製薬(株)研究所
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平井 瑛三
Shionogi Research Laboratories Shionogi & Co. Ltd.
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