Fluorometric Determination of an Inverse Agonist to Benzodiazepine Receptors S-135 in Monkey Plasma by High Performance Liquid Chromatography
スポンサーリンク
概要
- 論文の詳細を見る
A high performance liquid chromatographic method for the determination of an agent of benzodiazepine receptor inverse agonist (S-135) was developed. S-135 in monkey plasma, after its administration, was assayed using the method. S-135 is ionized in alkaline N, N-dimethylformamide (DMF), and fluoresces strongly at 490 nm on excitation at 290 nm. Extracted S-135 from a plasma sample was separated with an Asahipak GS-310H polyvinyl alcohol gel column and detected fluorometrically, with the eluate being mixed with a triethylamine-DMF mixture. S-135 in plasma is assayable with a coefficient of variation being less than 3.5% (n=11). The lower limit of the determination is 2 ng/ml. This method was applied as a highly sensitive and simple method to pharmacokinetic studies of S-135 after oral administration to cynomolgus monkeys.
- 公益社団法人日本薬学会の論文
- 1991-10-25
著者
-
北川 隆康
Shionogi Research Laboratories, Shionogi & Co., Ltd.,
-
北川 隆康
Shionogi Research Laboratories Shionogi & Co. Ltd.
-
北川 隆康
塩野義製薬(株)研究所
-
池西 利喜夫
Shionogi Research Laboratories, Shionogi & Co., Ltd.,
-
池西 利喜夫
Shionogi Research Laboratories Shionogi & Co. Ltd.
関連論文
- Application of Fluorescent Triazoles of Analytical Chemistry. IV. : Development of a New Fluorescent Probe for Basic Drug-α_1-Acid Glycoprotein Binding Measurement
- Studies on the Interaction of Pyridone Carboxylic Acids with Metals
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. VI. Ethereal Solvents Effect on the Lactim-Lactam Tautomerism
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. V. Lactim-Lactam Tautomerism in Ethereal Solvents
- Application of Fluorescent Triazoles to Analytical Chemistry. III. : Fluorescence Characteristics of 2-Phenylbenzotriazolyl-5-amine Derivatives as Fluorescent Probes
- Appication of Fluorecent Triazoles to Analytical Chemistry. II. : Fluorescent Derivatization of Carboxylic Acids
- Application of Fluorescent Triazoles to Analytical Chemistry. I. Determination of Aromatic Primary Amine with 2,4,6-Triaminopyrimidine as a Reagent
- Ion Pair Extraction of Trimethoprim with Sulfonphthalein Dyes
- A New Fluorometric Method for Latamoxef in Biological Materials Using 2,6-Diamino-3-nitrosopyridine
- Equilibrium Studies on 2-(5-Methylthien-3-yl)-2,5-dihydro-3H-pyrazolo[4,3-c]quinolin-3-one (S-135) in n-Octanol
- ユウロピウム錯体修飾タンパクを試薬とするイムノアッセイ
- Fluorometric Determination of an Inverse Agonist to Benzodiazepine Receptors S-135 in Monkey Plasma by High Performance Liquid Chromatography
- Protein Binding of Quinolonecarboxylic Acids. II. : Spectral Changes on the Interaction of Cinoxacin, Nalidixic Acid and Pipemidic Acid with Human and Rat Albumins
- Protein Binding of Quinolonecarboxylic Acids. I. Cinoxacin, Nalidixic Acid and Pipemidic Acid
- Gas Chromatographic Method for the Dethrmination of Fluoride Ion in Biological Samples. II. : Stability of Fluorine-Containing Drugs and Compounds in Human Plasma
- Gas Chromatographic Method for the Determination of Fluoride Ion in Biological Samples. I. : Fluoride Level in Monkey
- Analytical Studies on 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-3-dimethylcarbamoyl-5-glycylaminomethyl-1H-1,2,4-triazole Hydrochloride Dihydrate. III. High-Performance Liquid Chromatographic Method Applicable to Animal Feed(Analytical,Chemical)
- Analytical Studies on 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1,2,4-triazole Hydrochloride Dihydrate. II. : A Fluorometric Method Applicable to Animal Feed
- Analytical Studies on Isoxazoles. VI. Colorimetric Determination of Some Isoxazole Herbicides with p-Dimethylaminocinnamaldehyde
- Analytical Studies on Isoxazoles. V. Colorimetric Determination of Isouron and Its Isomer with p-Dimethylaminocinnamaldehyde
- Colorimetric Determination of 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1,2,4-triazole Hydrochloride Dihydrate with 3,5-Dibromosalicylaldehyde
- Colorimetric Determination of 4-Chloro-2-(o-chlorobenzoyl)-N-methyl-N^α-glycylglycinanilide with 3,5-Dibromosalicylaldehyde. II. Reaction Mechanism of Coloration
- Analytical Studies on Isoxazoles. IV. : Fluorometric Determination of Isouron
- 3,5-ジブロムサリチルアルデヒドを用いる4-クロロ-2-(o-クロロベンゾイル)-N-メチル-N^α-グリシルグリシンアニリドの比色定量法
- イソキサゾール類に関する分析化学的研究(第3報)イソウロンの比色定量
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. IV. Lactim-Lactam Tautomerism in Methanol, n-Butanol and n-Hexane
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. III. Photometric Titration in n-Butanol
- Acid-Base Equilibria of Aniline Derivatives in n-Butanol
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. II. Photometric Titration in Methanol
- Equilibrium Studies of 5-Substituted 4-Hydroxy-2-methylpyrimidines. I. The Ionization Constants in Aqueous Solution