Analytical Studies on 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-3-dimethylcarbamoyl-5-glycylaminomethyl-1H-1,2,4-triazole Hydrochloride Dihydrate. III. High-Performance Liquid Chromatographic Method Applicable to Animal Feed(Analytical,Chemical)
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概要
- 論文の詳細を見る
An ion-pair reversed-phase high-performance liquid chromatographic (HPLC) assay method was developed for the sleep inducer 1-(2-o-chlorobenzoyl-4-chlorophenyl)-3-dimethylcarbamoyl-5-glycylarninomethyl-1H-1,2,4-triazole hydrochloride dihydrate (rilmazafone, 1) in animal feed. This method is an ion-pair reversed-phase HPLC using an octadecylsilane chemically bonded silica gel column and dodecanesulfonic acid as an ion-pairing reagent. It can simultaneously assay drug 1 and its six degradation products. Dodecanesulfonic acid caused retention of three compounds (1,6 and 7) on the column through the ion-pair effect, with good separation from other neutral and acidic compounds. The drug was extracted from the feed. After clean-up, the extract was subjected to HPLC, with 1 being assayed with a coefficient of variation of less than 4% The degradation products can be simultaneously assayed with the same precision even when present at only 2-3 mg/kg. This method was used to confirm the stability of 1 in feed. An HPLC method to assay 1 alone was also established; it is more efficient than our previous fluorometric procedure.
- 公益社団法人日本薬学会の論文
- 1987-11-25
著者
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北川 隆康
Shionogi Research Laboratories, Shionogi & Co., Ltd.,
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北川 隆康
Shionogi Research Laboratories Shionogi & Co. Ltd.
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北川 隆康
塩野義製薬(株)研究所
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池西 利喜夫
Shionogi Research Laboratories, Shionogi & Co., Ltd.,
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池西 利喜夫
Shionogi Research Laboratories Shionogi & Co. Ltd.
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