Gas Chromatographic Method for the Dethrmination of Fluoride Ion in Biological Samples. II. : Stability of Fluorine-Containing Drugs and Compounds in Human Plasma

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Our gas chromatogrophic method established previusly has been modified to assay fluoride ion which is released from fluorine-containing compounds and drugs in human plasma. A strongly acidic medium was not necessarily required, though fluoride is usually derivatized to trimethylfluorosilane (TMFS) under such acidic conditions. Trimethylsiylation proceeded in pH 2.5 sulfosalicylate (SSA) buffer. Even labile fluorine-containing compounds were stable in SSA buffer, while they were degraded in a strong acid to form fluofide. SSA was effecitive for buffering HCl which was produced by the reaction of excess reagent, trimthylchlorosilane (TMCS), with H_2O. At the same time, SSA in plasma sample served as not only a deproteinizing agent but also a buffer agent for biological materials. By means of the present method, released fluoride in human plasma is assayable even in the presence of labile fluorine-containing compounds. More than 5 ng/ml of fluoride can be quantitated. α-Fluoro-β-alanine, 4-fluoroglutamic acid, 3-difluoroalanine and 5-(trifluoromethyl)uracil released fluoride in human plasma at 37℃ in vitro. The amount of fluoride increased with time. On the other hand, no fluoride was liberated from 5-fluorouracil of from compounds of drugs studied other than the above ones.
公益社団法人日本薬学会の論文
1988-02-25