Purification and Properties of Z Protein from Rabbit and Rat Liver
スポンサーリンク
概要
- 論文の詳細を見る
Z protein was purified from the rabbit and rat liver by fractionation with ammonium sulfate. The physico-chemical properties of Z protein was studied by SDS (sodium dodecyl sulfate) polyacrylamide gel electrophoresis, polyacrylamide gel electrofocusing, amino acid analysis, and sedimentation analysis. The molecular weight was determined to be 1.2×10^4 by gel filtration on Sephadex G-75 and 1.1×10^4 by SDS-polyacrylamide gel electrophoresis, and also 8872 and 8639 from amino acid analysis with the rabbit and rat liver, respectively. Z protein lacked tyrosine and tryptophan residues, and had an absorption maximum at 260-270 nm. The isoelectric point was at pH7.0. The sedimentation coefficient of Z protein was ca. 0.7S at a concentration of 0.04-0.06%. Physical and chemical properties of rabbit and ratliver Z protein were closely alike.
- 社団法人日本薬学会の論文
- 1977-04-25
著者
-
森口 郁生
School of Pharmaceutical Sciences, Kitasato University
-
梅山 秀明
School of Pharmaceutical Sciences, Kitasato University
-
梅山 秀明
School Of Pharmaceutical Sciences Kitasato University
-
森口 郁生
School Of Pharmaceutical Sciences Kitasato University
-
松下 泰雄
School of Pharmaceutical Sciences, Kitasato University
-
松下 泰雄
School Of Pharmaceutical Sciences Kitasato University
関連論文
- Furo[3,2-b]indole Derivatives. V. Synthesis and Structure-Activity Studies of 4-Substituted 2-(4-Methylpiperazinylcarbonyl)-6-trifluoromethylfuro[3,2-b]indole Derivatives with Analgesic and Antiinflammatory Activities(Medicinal Chemistry,Chemical)
- Furo[3,2-b]indole Derivatives. III. : Structure-Activity Studies of 4,6-Disubstituted N-(3-Piperidinopropyl)furo[3,2-b]indole-2-carboxamide Derivatives for Analgesic and Antiinflammatory Activities
- Synthesis of Human Renin Inhibitory Peptides, Angiotensinogen Transition-State Analogs Containing a Retro-Inverso Amide Bond
- Synthesis and Structure-Activity Relationships of Human Renin Inhibitors Designed from Angiotensinogen Transition State
- Hydrophobic Effect on the Protein-Ligand Interaction; Hydrophobic Field-Effect Index and Hydrophobic Correlation Index
- Structure-Activity Relationship of Fluorine-Containing Renin Inhibitory Peptides Based upon the Tertiary Structure of Human Renin
- NEW HUMAN RENIN INHIBITORY PEPTIDES : ANGIOTENSINOGEN TRANSITION-STATE ANALOGUES CONTAINING NOVEL LEU-VAL REPLACEMENT AND A RETRO-INVERSO AMIDE BOND
- A NEW METHOD FOR CALCULATING HYDROPHOBIC INTERACTION ENERGY IN THE BIOLOGICAL SYSTEM
- Correlation between Molecular Orbital Distributions in Drug-Receptor Interaction : Diels-Alder Reaction Systems and Dihydrofolate Reductase System(Physical,Chemical)
- Reproduction of ab Initio Electrostatic Potential with Classical Fractional Point Charges(Physical,Chemical)
- Electrostatic Interaction Energy and Solvent Accessibility in the Methotrexate-Reduced Nicotinamide Adenine Dinucleotide Phosphate-Dihydrofolate Reductase Ternary Complex(Medicinal Chemistry,Chemical)
- 薬物-受容体相互作用のグラフィック表現とドラッグデザインへの応用 (構造活性相関とドラッグデザイン)
- Electrostatic Potential Images of Drugs Targetting Dopamine Receptors
- A Molecular Orbital Study on Tetranactin-NH_4+ Complex
- A Molecular Orbital Study on the n-π^* Cotton Effect of ε-Caprolactam
- Molecular Orbital Study of Lactate Dehydrogenase
- Purification and Properties of Z Protein from Rabbit and Rat Liver
- In Vitro Binding of Sulfobromophthalein to Cytoplasmic Protein from Liver, Kidney, and Small Intestinal Mucosa of Rat and Rabbit
- A Molecular Orbital Study on the Effects of Substituents on the Proton Transfer from Ser-195 to His-57 in the Hydrolysis of α-Chymotrypsin
- Computer Screening and Visulization of Hydrophobic Core of Protein
- ELECTROSTATIC FORCES IN THE INHIBITION OF DIHYDROFOLATE REDUCTASE BY METHOTREXATE. A FIELD POTENTIAL STUDY
- CHARGE STATE OF HIS 57-ASP 102 COUPLE IN β-TRYPSIN : A MOLECULAR ORBITAL STUDY
- Molecular Orbital Study of Proton Transfer Energetics in the Active Site of Papain by Using Methanethiol-Imidazole-Formic Acid Complex as a Model
- Energy Decomposition Analyses of Diborane
- Molecular Orbital Study on the Structure and Barrier to Internal Rotation of Phosphine-Borane
- A Molecular Orbital Study on the Zinc-Water-Glu 270 System in Carboxypeptidase A
- Simulation of the Enzymatic Reaction of Dogfish M_4 Lactate Dehydrogenase : A Molecular Orbital Study on the Reactivity of Pyruvate
- Effects of the Hydrogen Bond between His 57 and Asp 102 on the Lone Pair Molecular Orbital of Nitrogen of His 57 in Serine Proteases
- Molecular Orbital Studies on the CH_3CN-BH_3,HCN-BH_3,CH_3NC-BH_3 and HNC-BH_3 Complexes
- Proton Migration in Proton Cryptate : A Molecular Orbital Study on a Proton Cryptate Model
- A Molecular Orbital Study on the (CH_3)_2O-BH_3 Donor-Acceptor Complex
- The Molecular Orbital Study on the Role of Hydrogen Bonding System in the Active Site of Serine Proteases
- A Molecular Orbital Study on the Approach of Hydride Ion to NAD^+ as a Coenzyme
- Structure-Activity Study of Antihypertensive 1,4-Dihydropyridine Derivatives Having Nitrooxyalkyl Moieties at the 3 and 5 Positions
- Synthesis of Antibacterial Agents having both Sulfanilamido- and Nitrofuryl- Groups in the Molecules. II.
- Synthesis of Antibacterial Agents having both Sulfanilamido- and Nitrofuryl- Groups in the Molecules. I
- Non-congeneric Structure-Pharmacokinetic Property Correlation Studies Using Fuzzy Adaptive Least-Squares : Volume of Distribution
- Comparison of Reliability of log P Values for Drugs Calculated by Several Methods
- Non-congeneric Structure-Pharmacokinetic Property Correlation Studies Using Fuzzy Adaptive Least-Squares : Oral Bioavailability
- Adsorption of Benzoic Acid Derivatives by Carbon Black from Aqueous Solution and Related Phenomena
- Protein Bindings. I. Binding of 2-(4'-Hydroxyphenylazo) benzoic Acid to Bovine Serum Albumin
- Studies on Selectivity of O-Methylation of Erythromycin Derivatives Based on Molecular Mechanics and Molecular Orbital Methods
- Structure-Activity Relationship Study of 6-O-Methylerythromycin 9-O-Substituted Oxime Derivatives
- Solvation Free Energies of Amino Acids Calculated by Molecular Dynamics/Free Energy Perturbation Method
- SOLUTION STRUCTURE OF HIV-1 PROTEASE-ALLOPHENYLNORSTATINE DERIVATIVE INHIBITOR COMPLEX OBTAINED FROM MOLECULAR DYNAMICS SIMULATION
- Comparison of the Functional-Link Net and the Generalized Delta Rule Net in Quantitative Structure-Activity Relationship Studies
- Fuzzy Adaptive Least Squares Applied to Structure-Activity and Structure-Toxicity Correlations
- Simple Method of Calculating Octanol/Water Partition Coefficient
- High Correlation between Hydrophobic Free Energy and MOlecular Surface Area Characterized by Electrostatic Potential
- Fuzzy Adaptive Least Squares and Its Use in Quantitative Structure-Activity Relationships
- Quantitative Structure-Activity Relationships for Calmodulin Inhibitors
- Quantitative Structure-Activity Relationships of O-Acyl Derivatives of Leucomycin for Antimicrobial and Ribosome-Binding Activities
- A Molecular Orbital Study on the Ternary Complex of Tetranactin, NH^+_4 and SCN^-
- A ab Initio Molecular Orbital Study of Molecular Interactions between Formic Acid and Ammonia
- Binding of Carprofen to Human and Bovine Serum Albumins
- Simulation of the Charge Relay Structure in Ribonuclease A
- Estimation of Hydrophobicity Based on the Solvent-Accessible Surface Area of Molecules
- A Molecular Orbital Study on the Side Chain Structures of Tyrosine and Phenylalanine
- A Molecular Orbital Study on the Complex between Aspartic Acid and Histidine in the Charge Relay Structure
- Ab Initio Molecular Orbital Studies on the Aspirin Solvolysis and Ester Hydrolysis
- Molecular Orbital Studies on Serine, Cysteine, and Modified Proteases
- Effects of Third Component on Hydrophobic and Hydrophilic Moities of Tryptophan in Aqueous Solution. Approach to Understanding of Denaturation of Globular Protein
- Effect of Third Component on Water Structure around Tryptophan in Aqueous Solution
- Application of Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate to the Studies on Protein Bindings and Protein-Mucopolysaccharide Interaction
- Studies on Protein Bindings utilizing Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate
- Binding of Drugs to Sera of Patients with Renal Disease and Liver Disease(Pharmaceutical)
- Mechanism of Adsorption of Phenols by Carbon Black from Aqueous Solution
- Analysis of Solubility Properties of L-Tryptophan in Aqueous Solution based on the Effects of Third Component on Optical Rotatory Dispersion and on Adsorption by Carbon Black
- Complexation of Chlorpromazine with Adenosine and Its Phosphates
- Effect of Water on Rate of Charge-Transfer Reaction of Aniline with Chloranil
- Effect of Water on Charge-Transfer Complexations
- Solvent Effects on π-π Charge-Transfer Complexations
- Spectral Changes of Various Dyes by Bovine Serum Albumin and by Organic Solvents
- Spectroscopic Studies on Molecular Interactions. VI. Mechanism of Metachromasy of 2-(4'-Hydroxyphenylazo) benzoic Acid by Serum Albumin
- Spectroscopic Studies on Molecular Interactions. V. Charge-Transfer Property and Enzymatic Acetylation of Aniline Derivatives
- Adsorption of Solute from the Solutions. IV. Adsorption of Benzoic Acids on Graphite
- Adsorption of Solute from the Solutions. V. Adsorption Rate of Cyanocobalamin on Talc
- Spectroscopic Studies on Molecular Interactions. IV. Charge-Transfer Properties and Antibacterial Activity of Sulfonamides
- Spectroscopic Studies on Molecular Interactions. III. Improvement of the Benesi-Hildebrand Method for the Determination of Equilibrium Constants
- Spectroscopic Studies on Molecular Interactions. II. Complexation between Polyvinylpyrrolidone and Iodine
- Spectroscopic Studies on Molecular Interactions. I. Complexations between Caffeine and Benzoic Acids
- Adsorption of Solute from the Solutions. III. Repression of Adsorption of Cyanocobalamin on Talc by Polyvinylpyrrolidone
- Adsorption of Solute from the Solutions. II. Competitive Adsorption of Cyanocobalamin with Pyridoxine and Thiamine on Talc
- AQUATIC TOXICITY. : A PREDICTIVE STRUCTURE-ACTIVITY MODEL
- Molecular Orbital Study on a Chlorine Anion Cryptate
- The Origin of the Internal Rotation Barrier of Borane Compounds
- Energy Decomposition Analysis of Borazane at the Molecular Orbital Level : Origin of the Internal Rotation Barrier of H_3X-YH_3
- A Molecular Orbital Study on the Solvolysis of Aspirin Derivatives and Acyl-α-chymotrypsin
- Protein Bindings. VI. Binding of Phenols to Bovine Serum Albumin
- Binding of Fluorescent 7-Amino-4-nitrobenzoxadiazole Derivatives to Bovine Serum Albumin
- An Equation for Ligand Binding to Protein over a Wide Range of Ligand Concentration
- Measurement of Protein Binding by Ultracentrifugation
- Quantitative Structure-Activity Studies. I. Parameters relating to Hydrophobicity
- Adaptive Least-Squares Classification applied to Structure-Activity Correlation of Antitumor Mitomycin Derivatives
- van der Waals Volume and the Related Parameters for Hydrophobicity in Structure-Activity Studies
- Protein Bindings. V. Binding of N^4-Acetylsulfonamides to Bovine Serum Albumin
- Protein Bindings. IV. Relations of an Index for Electronic Structure to Binding Constant with Serum Albumin and Bacteriostatic Activities of Sulfonamides
- Protein Bindings. III. Binding of Sulfonamides to Bovine Serum Albumin
- Use of van der Waals Volume in Structure-Activity Studies
- Protein Bindings. II. Binding of Aromatic Carboxylic Acids to Bovine Serum Albumin