Binding of Carprofen to Human and Bovine Serum Albumins
スポンサーリンク
概要
- 論文の詳細を見る
The binding of carprofen (CP) to human serum albumin (HSA) and bovine serum albumin (BSA) was compared using equilibrium dialysis method. The affinity of CP for the primary binding site was BSA>HSA. However, the number of primary binding sites(n_1) was 1.94 on HSA, considerably greater than that on BSA (0.79). The displacement of the binding of CP to HSA and BSA was studied in the presence of phenylbutazone (PB, site I marker), ibuprofen (IB, site II marker) or 2,3,5-triiodobenzoic acid (TIB, both site I and II marker). The binding of CP to HSA was altered by PB, IB and TIB, while the binding of CP to BSA was not changed by PB, although it was reduced by IB and TIB. These results suggested that, for the binding of CP, HSA has two major sites (site I and site II), whereas BSA has a single primary site (site II). The binding characteristics of 2-anthracenecarboxylic acid with HSA and BSA were found quite similar to those of CP. Thus, it seemed that long, planar compounds with a carboxyl group at one end can bind to site I and site II on HSA, but only to site II on BSA. The difference between HSA and BSA in the affinity of site I may be due to the difference in the basic and hydrophobic amino acid residues.
- 公益社団法人日本薬学会の論文
- 1994-04-15
著者
-
森口 郁生
School of Pharmaceutical Sciences, Kitasato University
-
森口 郁生
School Of Pharmaceutical Sciences Kitasato University
-
松下 泰雄
School of Pharmaceutical Sciences, Kitasato University
-
小比田 英機
School of Pharmaceutical Sciences, Kitasato University
-
松下 泰雄
School Of Pharmaceutical Sciences Kitasato University
-
小比田 英機
School Of Pharmaceutical Sciences Kitasato University
関連論文
- Furo[3,2-b]indole Derivatives. V. Synthesis and Structure-Activity Studies of 4-Substituted 2-(4-Methylpiperazinylcarbonyl)-6-trifluoromethylfuro[3,2-b]indole Derivatives with Analgesic and Antiinflammatory Activities(Medicinal Chemistry,Chemical)
- Furo[3,2-b]indole Derivatives. III. : Structure-Activity Studies of 4,6-Disubstituted N-(3-Piperidinopropyl)furo[3,2-b]indole-2-carboxamide Derivatives for Analgesic and Antiinflammatory Activities
- Comparison of Binding Characteristics of Human and Bovine Serum Albumins with Benzoates over a Wide Range of Concentration(Pharmaceutical)
- Electrostatic Potential Images of Drugs Targetting Dopamine Receptors
- A Molecular Orbital Study on Tetranactin-NH_4+ Complex
- A Molecular Orbital Study on the n-π^* Cotton Effect of ε-Caprolactam
- Purification and Properties of Z Protein from Rabbit and Rat Liver
- In Vitro Binding of Sulfobromophthalein to Cytoplasmic Protein from Liver, Kidney, and Small Intestinal Mucosa of Rat and Rabbit
- Structure-Activity Study of Antihypertensive 1,4-Dihydropyridine Derivatives Having Nitrooxyalkyl Moieties at the 3 and 5 Positions
- Synthesis of Antibacterial Agents having both Sulfanilamido- and Nitrofuryl- Groups in the Molecules. II.
- Synthesis of Antibacterial Agents having both Sulfanilamido- and Nitrofuryl- Groups in the Molecules. I
- Non-congeneric Structure-Pharmacokinetic Property Correlation Studies Using Fuzzy Adaptive Least-Squares : Volume of Distribution
- Comparison of Reliability of log P Values for Drugs Calculated by Several Methods
- Non-congeneric Structure-Pharmacokinetic Property Correlation Studies Using Fuzzy Adaptive Least-Squares : Oral Bioavailability
- Protein Bindings. I. Binding of 2-(4'-Hydroxyphenylazo) benzoic Acid to Bovine Serum Albumin
- Studies on Selectivity of O-Methylation of Erythromycin Derivatives Based on Molecular Mechanics and Molecular Orbital Methods
- Structure-Activity Relationship Study of 6-O-Methylerythromycin 9-O-Substituted Oxime Derivatives
- Solvation Free Energies of Amino Acids Calculated by Molecular Dynamics/Free Energy Perturbation Method
- SOLUTION STRUCTURE OF HIV-1 PROTEASE-ALLOPHENYLNORSTATINE DERIVATIVE INHIBITOR COMPLEX OBTAINED FROM MOLECULAR DYNAMICS SIMULATION
- Comparison of the Functional-Link Net and the Generalized Delta Rule Net in Quantitative Structure-Activity Relationship Studies
- Fuzzy Adaptive Least Squares Applied to Structure-Activity and Structure-Toxicity Correlations
- Simple Method of Calculating Octanol/Water Partition Coefficient
- High Correlation between Hydrophobic Free Energy and MOlecular Surface Area Characterized by Electrostatic Potential
- Fuzzy Adaptive Least Squares and Its Use in Quantitative Structure-Activity Relationships
- Quantitative Structure-Activity Relationships for Calmodulin Inhibitors
- Quantitative Structure-Activity Relationships of O-Acyl Derivatives of Leucomycin for Antimicrobial and Ribosome-Binding Activities
- Binding of Carprofen to Human and Bovine Serum Albumins
- Estimation of Hydrophobicity Based on the Solvent-Accessible Surface Area of Molecules
- Application of Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate to the Studies on Protein Bindings and Protein-Mucopolysaccharide Interaction
- Studies on Protein Bindings utilizing Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate
- Binding of Drugs to Sera of Patients with Renal Disease and Liver Disease(Pharmaceutical)
- Complexation of Chlorpromazine with Adenosine and Its Phosphates
- Effect of Water on Rate of Charge-Transfer Reaction of Aniline with Chloranil
- Effect of Water on Charge-Transfer Complexations
- Solvent Effects on π-π Charge-Transfer Complexations
- Spectral Changes of Various Dyes by Bovine Serum Albumin and by Organic Solvents
- Spectroscopic Studies on Molecular Interactions. VI. Mechanism of Metachromasy of 2-(4'-Hydroxyphenylazo) benzoic Acid by Serum Albumin
- Spectroscopic Studies on Molecular Interactions. V. Charge-Transfer Property and Enzymatic Acetylation of Aniline Derivatives
- Adsorption of Solute from the Solutions. IV. Adsorption of Benzoic Acids on Graphite
- Adsorption of Solute from the Solutions. V. Adsorption Rate of Cyanocobalamin on Talc
- Spectroscopic Studies on Molecular Interactions. IV. Charge-Transfer Properties and Antibacterial Activity of Sulfonamides
- Spectroscopic Studies on Molecular Interactions. III. Improvement of the Benesi-Hildebrand Method for the Determination of Equilibrium Constants
- Spectroscopic Studies on Molecular Interactions. II. Complexation between Polyvinylpyrrolidone and Iodine
- Spectroscopic Studies on Molecular Interactions. I. Complexations between Caffeine and Benzoic Acids
- Adsorption of Solute from the Solutions. III. Repression of Adsorption of Cyanocobalamin on Talc by Polyvinylpyrrolidone
- Adsorption of Solute from the Solutions. II. Competitive Adsorption of Cyanocobalamin with Pyridoxine and Thiamine on Talc
- AQUATIC TOXICITY. : A PREDICTIVE STRUCTURE-ACTIVITY MODEL
- Protein Bindings. VI. Binding of Phenols to Bovine Serum Albumin
- Binding of Fluorescent 7-Amino-4-nitrobenzoxadiazole Derivatives to Bovine Serum Albumin
- An Equation for Ligand Binding to Protein over a Wide Range of Ligand Concentration
- Measurement of Protein Binding by Ultracentrifugation
- Quantitative Structure-Activity Studies. I. Parameters relating to Hydrophobicity
- Adaptive Least-Squares Classification applied to Structure-Activity Correlation of Antitumor Mitomycin Derivatives
- van der Waals Volume and the Related Parameters for Hydrophobicity in Structure-Activity Studies
- Protein Bindings. V. Binding of N^4-Acetylsulfonamides to Bovine Serum Albumin
- Protein Bindings. IV. Relations of an Index for Electronic Structure to Binding Constant with Serum Albumin and Bacteriostatic Activities of Sulfonamides
- Protein Bindings. III. Binding of Sulfonamides to Bovine Serum Albumin
- Use of van der Waals Volume in Structure-Activity Studies
- Protein Bindings. II. Binding of Aromatic Carboxylic Acids to Bovine Serum Albumin