Comparison of Binding Characteristics of Human and Bovine Serum Albumins with Benzoates over a Wide Range of Concentration(Pharmaceutical)

概要

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The binding parameters of human serum albumin(HSA) and bovine serum albumin(BSA) with 14 benzoate derivatives and 2-naphthoate were determined by means of the ultracentrifugationmethod and the three-parameter equation previously proposed. The multiple regresssion analysis ofthe binding parameters revealed that the physico-chemical factors influencing the parameter a,which takes account of the increase in the binding capacity of albumin, were different in partbetween HSA and BSA. Moreover, the mean of the a values was greater with HSA than with BSA.This may indicate that HSA has a more flexible structure, and this may be consistent with lowercontents of a-helix and β-sheet in the HSA molecule. Such differences were not recognized in thebinding affinity of the serum albumins.
社団法人日本薬学会の論文
1987-06-25