EXPERIMENTAL STUDY FOR THE TOXICOLOGICAL EFFECTS OF SVATE-3 ON RAT LIVER : OBSERVATION BY LIGHT AND ELECTRON MICROSCOPE AND BY ENZYME HISTOCYTOCHEMISTRY

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In the present study, we have done an acute toxicological experiment on the rat liver by administration of SVATE-3 in clinical doses (high and low dose, respectively) for 3 weeks. By using morphological methods, light and electron microscopy, enzyme histocytochemistry in light and electron microscope and biochemistry, we have observed the histological structure and ultrastructure of rat livers as well as the activity and distribution of the index enzymes for the liver cell membrane such as alkaline phosphatase, 5'-nucleotidase, Mg-adenosine triphosphatase and glucose-6-phosphatase for liver cell organelle: endoplasmic reticulum. Furthermore, the biochemical quantitation has been given. The results show that there is no toxic damage to the histological structure of rat liver by administration of SVATE-3 in the acute toxicological experiments. There is no obvious change of histocytochemical enzyme activity and distribution of these 4 types of index enzymes. All these suggest that the application of SVATE-3 within clinical doses shows no toxic damage to rat liver.
日本組織細胞化学会の論文