The Anticancer Activities of Wogonin in Murine Sarcoma S180 both in Vitro and in Vivo(Pharmacology)

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概要

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• The anticancer effects of wogonin on murine sarcoma S180 both in vitro and in vivo were investigated, and its pro-apoptotic molecular mechanism was further studied. Wogonin treatment resulted in significant inhibition of S180 cells in a concentration-dependent manner detected by MTT assay. The IC_<50> value for 48h was (7.37±1.53)×10^<-5>M. Typical morphological changes and apoptosis bleb phenomenon in S180 cells exposed to wogonin were distinctly observed by the inverted light microscope and the fluorescence microscope, respectively. According to protocols of transplanted tumor research, mice were transplanted with tumor cells S180. The weight of tumor and the peripheral leucocyte count were observed after the treatment of wogonin. The significant suppression of tumor growth was observed, and the peripheral leucocyte count of S180-bearing mice remained no significant changes compared with control group. After the treatment of 40mg/kg wogonin, the inhibitory rate of tumor weight was 53.01%. Additional DNA fragmentation assay showed that wogonin induced apoptosis on murine sarcoma S180 tissue. RT-PCR results indicated that the increasing mRNA levels of bax and p53 and the decreasing mRNA level of bcl-2 were induced by wogonin. Western-blot assay showed that the increasing protein level of bax and the decreasing protein level of bcl-2 were induced by wogonin. Collectively, wogonin could induce apoptosis in murine sarcoma S180 thereby inhibiting the tumor growth both in vitro and in vivo. The pro-apoptotic effects might be related to the improvement of mRNA level of p53, the improvement of mRNA and protein levels of bax, and the reduction of mRNA and protein levels of bcl-2.

• 社団法人日本薬学会の論文
• 2006-06-01

著者

• WANG Wei

  Department of Mechanical Engineering, Chiba University

• YU Jun

  Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University

• Yu Jun

  Department Of Electronic Science & Technology Huazhong University Of Science & Technology

• Yu Jun

  Department Of Physiology China Pharmaceutical University

• You Qi-dong

  Jiangsu Key Lab. Of Carcinogenesis And Intervention (china Pharmaceutical University) People's

• You Qi-dong

  Jiangsu Key Laboratory Of Carcinogenesis And Intervention China Pharmaceutical University

• GUO Qing-Long

  Department of Physiology, China Pharmaceutical University

• YOU Qi-Dong

  Department of Medicinal Chemistry, China Pharmaceutical University

• ZHANG Kun

  Department of Physiology, China Pharmaceutical University

• YANG Yong

  Department of Physiology, China Pharmaceutical University

• LIU Wei

  Department of Physiology, China Pharmaceutical University

• ZHAO Li

  Department of Physiology, China Pharmaceutical University

• GU Hong-Yan

  Department of Physiology, China Pharmaceutical University

• HU Yang

  Department of Physiology, China Pharmaceutical University

• TAN Zi

  Department of Chemistry and Biochemistry, Florida International University

• WANG Xiao-Tang

  Department of Chemistry and Biochemistry, Florida International University

• Gu Hong-yan

  中華人民共和国

• Gu Hong-yan

  Jiangsu Key Laboratory Of Carcinogenesis And Intervention China Pharmaceutical University

• Tan Zi

  Department Of Chemistry And Biochemistry Florida International University

• Zhao Li

  中華人民共和国

• Zhao Li

  Department Of Epidemiology Tongliao Center For Disease Prevention And Control

• Zhang Kun

  Jiangsu Key Laboratory Of Carcinogenesis And Intervention China Pharmaceutical University

• Guo Q‐l

  Jiangsu Key Laboratory Of Carcinogenesis And Intervention China Pharmaceutical University

• Guo Qing-long

  Department Of Physiology No. 24 Tong Jia Xiang China Pharmaceutical University

• Wang Xiao-tang

  Department Of Chemistry And Biochemistry Florida International University

• Liu Wei

  Department Of Geriatric Medicine Kanazawa Medical University:respiratory Department Of Zhong Nan Hos

• Yang Yong

  Jiangsu Key Laboratory Of Carcinogenesis And Intervention China Pharmaceutical University

• Hu Yang

  Department Of Physiology China Pharmaceutical University

• Zhao L

  Saitama Univ. Saitama

• Zhao Li

  Department Of Endocrinology Metabolism And Nephrology Kochi Medical School Kochi University

• Zhao Li

  Faculty Of Information And Computer Sciences Toyohashi University Of Technology

• Liu Wei

  Department Of Analytical Chemistry Shenyang Pharmaceutical University

• Wang Wei

  Department Of General Surgery Shanghai Chang Zheng Hospital Second Military Medical University

• Wang Wei

  Department Of Cardiology Shanghai Xuhui Central Hospital

• Liu Wei

  Department Of Physiology China Pharmaceutical University

• Wang Wei

  Department Of Physiology China Pharmaceutical University

• Wang Wei

  Department Of Biology Graduate School Chinese Academy Of Sciences Beijing

• Wang Wei

  Department Of Biochemistry Hainan Medical College

• Wang Wei

  Department of Anesthesiology, China-Japan Friendship Hospital, the Chinese Ministry of Health, Beijing, China

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