USE OF AMP (2-AMINO-2-METHYL-1-PROPANOL) BUFFER FOR HISTOCHEMICAL DEMONSTRATION OF ALKALINE PHOSPHATASE ACTIVITY
スポンサーリンク
概要
- 論文の詳細を見る
AMP (2-amino-2-methyl-1-propanol) buffer was tried for the histochemical detection of alkaline phosphatase (ALPase) activity by the lead citrate method. The incubation medium tested was as follows: 170-350mM AMP, 20mM sodium β-glycerophosphate, 3 .9mM magnesium sulfate, 2.0mM lead citrate and 8% sucrose; pH was adjusted to 9.2. By using this medium, ALPase activity in the liver was found in the sinusoidal and lateral surface of hepatocytes as well as in the bile canalicular surface, which was the only positive site by the original lead citrate method using Tris buffer. Moreover, the reaction product (lead phosphatc) appeared finer than when using the Tris method. By quantitating ALPase activity biochemically, higher activity was found with AMP than with Tris. Apparently, this new incubation medium with AMP is suitable for detecting weak ALPase activrity.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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Shi Yu
Department Of Anatomy Faculty Of Medicine Kyoto University:department Of Histoembryology China Medic
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Shi Yu
中華人民共和国
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Shi Y
Department Of Histoembryology China Medical University:no. 2 Laboratory Of Electron Microscopy China
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Shi Yu-xiu
Department Of Anatomy Faculty Of Medicine Kyoto University:department Of Histoembryology China Medic
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ARAKI NOBUKAZU
Department of Anatomy, Faculty of Medicine, Kyoto University
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FUJIMOTO TOYOSHI
Department of Anatomy, Faculty of Medicine, Kyoto University
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Fujimoto T
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Fujimoto Toyoshi
Department Of Anatomy And Cell Biology Gunma University School Of Medicine
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Fujimoto Toyoshi
Department Of Anatomy & Cell Biology Nagoya University Graduate School Of Medicine
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Araki N
Department Of Histology And Cell Biology School Of Medicine Kagawa University
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Araki Nobukazu
Department Of Anatany Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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