DOUBLE STAINING PROCEDURE FOR HISTOCHEMICAL LOCALIZATION OF ALCOHOL AND ALDEHYDE DEHYDROGENASE ACTIVITIES IN THE MOUSE LIVER
スポンサーリンク
概要
- 論文の詳細を見る
The double staining procedure using both the nitroblue tetrazolium (NBT) and copper ferrocyanide methods was presented for the simultaneous localization of two different dehydrogenase activities in a single histologic section. Sections of normal mouse liver were stained for alcohol dehydrogenase (ADH) activity using the NBT method first, then stained for aldehyde dehydrogenase (ALDH) activity using the copper ferrocyanide method. However, the formazan showing ADH activity must chelate metal ion (Cu^<2+>, Co^<2+>, or Ni^<2+>) prior to staining for ALDH activity. Both the blue metal-formazan complex showing ADH and brown copper ferro-cyanide showing ALDH activities in a single liver section were localized mainly in the perivenous zone of the hepatic lobule. In addition, ALDH activity is always localized in the zone of the lobule where ADH activity is present.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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時安 太久麿
Department of Legal Medicine, Kansai Medical University
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時安 太久麿
Department Of Legal Medicine Kansai Medical University
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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WATABIKI TOSHIMITSU
Department of Legal Medicine, Kansai Medical University
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TOKIYASU TAKUMA
Department of Legal Medicine, Kansai Medical University
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ISHIDA NORIAKI
Department of Legal Medicine, Kansai Medical University
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Ishida Noriaki
Department Of Legal Medicine Kansai Medical University
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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綿引 利充
Department Of Legal Medicine Kansai Medical University
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Watabiki Toshimitsu
Department Of Legal Medicine Kansai Medical University:department Of Anatomy Kyoto University Facult
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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