ULTRACYTOCHEMICAL LOCALIZATION OF CA^<++>-ATPASE ACTIVITY IN THE EPITHELIAL CELLS OF GUINEA PIG CILIARY BODY
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概要
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Ca^<++>-activated adenosine triphosphatase (Ca^<++>-ATPase) activity in the epithelial cells of guinea pig ciliary body was demonstrated histo- and cytochemically with the use of a newly developed method (Ando et at., 1981). In nonpigment ciliary epithelial cells fixed for 60 min in paraformaldehyde, intense reaction was observed on basolateral plasmalemma and mitochondria. In contrast, an intense reaction was localized only on mitochondria and gap junctions but not on the plasmalemma in the pigment epithelial cells. From the controls, this reaction was completely Ca^<++>- and ATP-dependent. The activity in fixed tissue with a mixture of paraformaldehyde and glutaraldehyde still remained moderately on the plasmalemma, but was completely abolished on mitochondria and gap junctions. The activity in the tissue after incubation in the medium with 2 mM or 3 mM Ca^<++> concentration remained on the plasmalemma but almost disappeared from the mitochondria and gap junctions. Utilization of 1 mM or lower Ca^<++> concentration failed to show enzyme activity. When tissue was incubated in the medium containing both 5 or 10 mM Ca^<++> and Mg^<++>, the reaction products on the mitochondria were still intense. However, the activity on the plasmalemma was markedly weakened. When Mg^<++> were used instead of Ca^<++>, a distinct reaction was also found on mitochondria, but not on the plasmalemma. Our results clearly revealed that Ca^<++>-ATPase activity was localized on the plasmalemma, mitochondria and gap junctions in the ciliary epithelium. It is noteworthy that a much more intense reaction existed on the basolateral plasmalemma which has been suggested to play an important role for aqueous humor secretion facing the posterior chamber in the nonpigment epithelium.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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UENO SATOKI
Department of Ophthalmology, Faculty of Medicine, Kyoto University
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Ueno Satoki
Departments Of Ophthalmology And Anatomy Faculty Of Medicine Kyoto University
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Ueno Satoki
Department Of Ophthalmology Faculty Cf Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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YASUBUCHI YUKIO
Departments of Ophthalmology and Anatomy, Faculty of Medicine, Kyoto University
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TSUKAHARA ISAMU
Departments of Ophthalmology and Anatomy, Faculty of Medicine, Kyoto University
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Yasubuchi Yukio
Departments Of Ophthalmology And Anatomy Faculty Of Medicine Kyoto University
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Tsukahara Isamu
Departments Of Ophthalmology And Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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