CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE IN THE RABBIT OVARIAN FOLLICLE

概要

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The different cell layers in the follicle wall in rabbit ovaries were examined for acid phosphatase activity during the ovulatory process. The tissue was taken before the process began and at 5, 7, 9, 10 and 11 hr after it was induced by human chorionic gonadotropin. The ovaries were perfused with a glutaraldehyde fixative, cut with a microslicer, incubated for 60 min at 37℃ in Gomori's acid phosphatase medium, and then routinely prepared for light and electron microscopy. Control tissue, which was incubated with the acid phosphatase inhibitor, sodium fluoride, did not reveal any enzyme activity. The results show acid phosphatase reaction product in all the major cell types in the follicle wall. The enzyme is located mainly in lysosomes and Golgi apparatus. It is not present in the electron-dense granules which are characteristic of the cytoplasm of surface epithelial cells. The acid phosphatase in the thecal layers of the follicle appears to be localized, not only in lysosomes and Golgi apparatus, but also in the multivesicular structures which are present in the thecal fibroblasts of this tissue. Collectively, the data indicate that acid phosphatase and other lysosomal enzymes may contribute, at least in part, to the hydrolytic degradation of follicular connective tissue during ovulation.
日本組織細胞化学会の論文