APPLICATION OF ANALYTICAL COLOR FLUORESCENCE ELECTRON MICROSCOPY TO BIOMEDICAL FIELD: II. CHOLESTEROL ESTER IN RAT ADRENAL CORTEX

概要

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Rat adrenal glands under several different conditions were observed under the analytical color fluorescence electron microscope (ACFEM) to elucidate the origin of the cathodoluminescence (CL) of adrenocortical cells. In the control group, blue-green CL which has two peak wavelength at 320 nm and 430 nm was observed to be emitted from lipid droplets of adrenocortical cells. When hypocholesterolemic drugs, 17α-ethinyl estradiol and 4-aminopyrazolo-pyrimidine, were administered, CL of 320 nm was observed to disappear in all three zones of the adrenal cortex. On the contrary, in hypophysectomized rat adrenal cortices, CL of 320 nm significantly increased, and that of 430 nm diminished. In the ACTH-treated group, CL of 320 nm in the zonae fasciculata and reticularis was observed to fade up to 2 hr after injection, while that in the zona glomerulosa remained. These results suggest that CL of 320 nm in adrenocortical cells is derived from cholesterol ester stored for steroidogenesis, and showed that the CL analysis using the ACFEM in combination with the cryo-SEM method is superior to conventional histochemical technique in simplicity, specificity, and preservation of ultrastructure.
日本組織細胞化学会の論文