CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE IN NORMAL AND CATARACTOUS RAT LENSES
スポンサーリンク
概要
- 論文の詳細を見る
Cytochemical localization and biochemical quantification of acid phosphatase (ACPase) activity were demonstrated in order to investigate the possible role of lysosomal enzymes in the process of cataract formation. ICR-strain and Wistar-strain rat (for the control experiment) were employed as experimental animals. Lenses of both groups of rats at 7 weeks and 16 weeks of age were fixed in 2% glutaraldehyde, 2% formaldehyde in 0.1 M cacodylate buffer. After rinsing overnight in the same buffer, 20 μm sections were made and incubated at 37℃ in a modified Gomori's medium. ACPase activity was also assayed biochemically using p-nitrophenyl phosphate as substrate. In all lenses, ACPase activity was detected mainly in the epithelium. In the anterior and posterior superficial cortex, ACPase activity was infrequently detected within lysosomes and intercellular spaces were free of reaction products in lenses of control rats. In pre-cataractous lenses (7-week-old ICR-strain rats), ACPase activity was generally more intense than that in lenses of control rats. Interestingly, reaction products were scattered in the posterior superficial cortical layer adjacent to the capsule. By contrast, reaction products in advanced cataractous lenses (16-week-old ICR-strain rats) were scarcely detected in the posterior region which was highly damaged. These results were congruent with the level of ACPase activity assayed biochemically. From the fact that there was not a continuous increase of ACPase activity, we inferred that the increase of ACPase activity might not be a direct initiator of cataract formation in this rat.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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Tanaka Toshikazu
Department of Cardiology, Okazaki City Hospital
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Sakai M
Kyushu Univ. Fukuoka Jpn
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Sakai M
Department Of Anatomy Faculty Of Medicine Kyoto University
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Tanaka Toshikazu
Department Of Anatomy Faculty Of Medicine Kyoto University
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Tanaka Takaaki
Department Of Oral Surgery Asahi University School Of Dentistry
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Sakai Masahiro
Department Of Anatomy Faculty Of Medicine Kyoto University
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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Tanaka T
Department Of Oral Anatomy And Cell Biology Graduate School Of Dental Sciences Kyushu University
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Tanaka Tomoo
Division Of Basic Medical Sciences Tokai University School Of Medicine
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Sakai M
Laboratory Of Forest Policy Division Of Forest Environment And Management Sciences Department Of For
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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