FODRIN IS LOCALIZED IN THE CYTOPLASM OF KERATINOCYTES CULTURED IN LOW CALCIUM MEDIUM : IMMUNOELECTRON MICROSCOPIC STUDY
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概要
- 論文の詳細を見る
Distribution of fodrin in a mouse keratinocyte cell line, Pam 212, was studied by two novel techniques of immunogold electron microscopy. Firstly, by utilizing ultra-small (1 nm) gold-conjugated antibody and silver enhancement in the pre-embedding procedure, the difference of fodrin distribution in three culture conditions was shown in a semiquantitative manner. Fodrin was observed throughout the cytoplasm in cells cultured in low calcium (LC: 0.06 mM) medium, but it was concentrated beneath the plasma membrane in cells in standard calcium (SC: 1.87 mM); when cells in LC medium were treated with 10 ng/ml 12-o-tetradecanoylphorbol-13-acetate (TPA) for two hr, the protein became dense near the plasma membrane. Secondly, by treating the immunogold-labeled sample by the dry-blowing method (Araki, N. and Ogawa, K.: Acta histochem. cytochem. 19; 31-37, 1986), fodrin was observed along actin-like microfilaments in Pam 212 cells in LC medium. After treatment with 30 μM cytochalasin B for one hr, the labeling became confined to an amorphous substance which is likely to be composed of depolymerized actin. The present study showed the distributional change of fodrin at the ultrastructural level: in IC cells fodrin was mostly in the cytoplasm and at least some were associated with actin filaments; 'calcium switch' produced its redistribution to the plasma membrane. In addition, since TPA was revealed to cause a similar result as 'calcium switch', phosphorylation of fodrin molecule might be causally related to the rearrangement.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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IMAMURA Sadao
Department of Dermatology, Faculty of Medicine, Kyoto University
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YONEDA Kozo
Department of Dermatology, Akita University School of Medicine
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FUJIMOTO TOYOSHI
Department of Anatomy, Faculty of Medicine, Kyoto University
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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Fujimoto T
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Fujimoto Toyoshi
Department Of Anatomy And Cell Biology Gunma University School Of Medicine
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Fujimoto Toyoshi
Department Of Anatomy & Cell Biology Nagoya University Graduate School Of Medicine
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Yoneda Kozo
Department Dermatology Faculty Of Medicine Kyoto University
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Yoneda K
Kyoto Univ. Kyoto Jpn
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Imamura S
Department Of Dermatology Faculty Of Medicine Kyoto University
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Imamura Sadao
Department Of Dermatology Faculty Cf Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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