ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT p-NITROPHENYLPHOSPHATASE ACTIVITY IN GUINEA PIG RETINA : II. NEURONS AND MULLER CELLS
スポンサーリンク
概要
- 論文の詳細を見る
Ouabain-sensitive, K-dependent p-nitrophenylphosphatase (K-NPPase) activity, which reflects the second dephosphorylative step of Na-K-ATPase, was investigated histochemically under light and electron microscopes in neurons and Muller cells of the normal adult guinea pig retina with the newly developed one step lead citrate method (Mayahara et al., 1980). In guinea pig retina fixed for 5 min in a mixture of 2% paraformaldehyde and 0.25% glutaraldehyde, electron dense reaction precipitates indicating K-NPPase activity were observed on plasma membranes of neurons and Muller cells. The most intensive K-NPPase reaction products were evident in the post-synaptic membranes of neurons in the outer plexiform layer, presynaptic membranes of neurons and post-synaptic membranes of ganglion cells and in the processes of Muller cells in the inner plexiform layer. This reaction was almost completely abolished by 10 mM ouabain or elimination of K. The activity was also substrate-dependent, and completely inhibited by PCMB-S or preheating. It is noteworthy that another p-nitrophenylphosphatase activity which is ouabain-insensitive, Levamisole-resistant and K-independent was recognized on the endoplasmic reticulum, Golgi apparatus and nuclear envelope solely inMuller cells. This activity was also substrate-dependent, and completely inhibited by PCMB-S or preheating. Mg-ATPase activity was observed only on the synaptic membranes of neurons and ganglion cells in the inner plexiform layer. However, this reaction could be neither inhibited by ouabain nor activated by K. Non-specific alkaline phosphatase activity was not demonstrated on any part of the plasma membranes of neurons, ganglion cells or Muller cells.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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MAYAHARA Hiroshi
Department of Radiology, Hyogo Ion Beam Medical Center
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Ueno Satoki
Departments Of Ophthalmology And Anatomy Faculty Of Medicine Kyoto University
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Mayahara Hiroshi
Departments Of Ophthalmology And Anatomy Kyoto University
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Mayahara Hiroshi
Department Of Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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TSUKAHARA ISAMU
Departments of Ophthalmology and Anatomy, Faculty of Medicine, Kyoto University
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UENO SATOKI
Faculty of Medicine, Kyoto University
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TSUKAHARA ISAMU
Faculty of Medicine, Kyoto University
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Tsukahara Isamu
Departments Of Ophthalmology And Anatomy Faculty Of Medicine Kyoto University
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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