THE THREE-DIMENSIONAL STRUCTURE OF THE GOLGI COMPLEX IN CULTURED FIBROBLASTS. AN ULTRACYTOCHEMICAL STUDY WITH THIN AND THICK SECTIONS (PHYSIOLOGY AND PATHOLOGY OF LYSOSOMES)

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The three-dimensional structure of the Golgi complex in human and mouse cultured fibroblasts was investigated ultracytochemically by demonstrating the acid phosphatase (ACPase) activity in the Golgi complex and observing ultrathin or thick (0.2-3 μm) sections with a conventional (80 or 100 KV) or high voltage (1,000 KV) electron microscope. The stereoscopic observation of stereo-pair pictures taken by tilting the thick sections with a goniometer has helped the understanding of the three-dimensional structure of the Golgi complex. The ACPase activity is positive in three different cross-sectional views of the Golgi complex: 1) the cisternal part, which is composed of three to several cisternae arranged in parallel; 2) the polygonal array of thin (75 um) tubules, which may be the face view of a Golgi cisterna; and 3) the reticular part, which is located at the inner (mature) face of the Golgi complex and is composed of flat portions and thick (100-160 nm) tubular portions. The reticular part of the Golgi complex may correspond to the GERL in other cells (16, 19, 21). The continuity of the reticular part of the Golgi complex (possibly GERL) throughout the cell was clearly demonstrated as a result of the use of thick sections cut parallel to the substratum, which enabled us to observe the whole Golgi complex system in a cell.
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