CYTOCHEMICAL DEMONSTRATION OF LECTIN BINDING SITES IN ROD PHOTORECEPTOR CELLS AND INTERPHOTORECEPTOR MATRIX OF RAT RETINAS BY LABELING FROZEN THIN-SECTIONS WITH VARIOUS LECTINS
スポンサーリンク
概要
- 論文の詳細を見る
We examined lectin binding sites in rod photoreceptor (RP) cells and interphotoreceptor matrix (KPM) of Wistar rat retinas in situ using frozen thin-sections reacted with various labeled lectins. Neuraminidase digestion was employed to study the glycosyl residues next to the terminal sialic acid. Lectins used in this study are as follows: concanavalin A (Con A), wheat germ agglutinin (WGA), Maclura pomifera agglutinin (MPA), peanut agglutinin (PNA) and Dolichos biflorus agglutinin (DBA). We also used isolated rod outer segments to study MPA-binding sites of the plasma membrane of the rod outer segment (ROS). In fluorescence micrographs of Con A- or WGA-fluorescence isothiocyanate (FITC) reacted retina, an intense staining was observed in the ROS and a meshwork-like staining was observed in the rod inner segment (RIS) and the outernuclear layer. Electron microscopic observations of frozen thin-sections labeled with Con A- or WGA-ferritin revealed that these ligands were bound to disk membranes of the ROS, membranous structures of the RIS, entire plasma membranes of the RP cells, and the KPM. In MPA-FITC reacted retina, an intense staining inthe ROS and the RIS showed a linear pattern. MPA-ferritin particles were bound to plasma membranes of the ROS and RIS, and to the IPM. In electron micrographs of isolated rod outer segments labeled with MPA-ferritin, MPA-bindings to the plasma membrane were greatly reduced, which suggest MPA was mainly bound to the IPM. No PNA- or DBA-labeling was observed in either RP cells or KPM. Following neuraminidase digestion, WGA-labeling was reduced and PNA-labeling appeared complementarily in the plasma membrane of the ROS and RIS, and the KPM.
- 日本組織細胞化学会の論文
著者
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Ogawa Kazuo
Department of Cardiology, Jikei University
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Kitaoka Takashi
Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki Un
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HONDA Yoshihito
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine
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UENO SATOKI
Department of Ophthalmology, Faculty of Medicine, Kyoto University
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Ueno Satoki
Department Of Ophthalmology Faculty Cf Medicine Kyoto University
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Ueno S
Kyoto Univ. Kyoto Jpn
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Ueno S
Department Of Ophthalmology Faculty Of Medicine Kyoto University
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Honda Y
Department Of Ophthalmology Faculty Cf Medicine Kyoto University
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Honda Yoshihito
Department Of Ophthalmology And Visual Science Graduate School Of Medicine Kyoto University
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FUJIMOTO KAZUSHI
Department of Anatomy, Faculty of Medicine, Kyoto University
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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Fujimoto K
Department Of Biochemistry Meikai University School Of Dentistry
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Fujimoto Kazushi
Department Of Anatomy And Neurobiology Graduate School Of Medicine Kyoto University
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Kitaoka Takashi
Department Of Anatomy Faculty Cf Medicine Kyoto University
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Kitaoka T
Nagasaki Univ. Nagasaki Jpn
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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Fujimoto Kazushi
Section Of Physiological Anatomy Fukui Prefectural University Faculty Of Nursing And Welfare Laborat
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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Honda Yoshihito
Department Of Ophthalmology Faculty Of Medicine Kyoto University
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Kitaoka Takashi
Department of Anatomy 2, Toyama Medical and Pharmaceutical University Faculty of Medicine
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