DIFFERENCES BETWEEN THE IN VITRO AND IN VIVO HISTOCHEMISTRY : ELECTRON MICROSCOPIC FEATURES OF POLYGLUCOSE PARTICLES HISTOCHEMICALLY SYNTHESIZED IN VITRO OR IN VIVO

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Histochemical techniques for glycogen synthetase and phosphorylase were applied to the living chick retina. Polyglucose particles which were histochemically synthesized in the paraboloid of the accessory cone by either enzyme in vivo were larger in size and better stainable with lead citrate than those in vitro. The retina incubated in the medium in vitro showed swelling of the paraboloid. The cytoplasmic matrices of the paraboloid were expanded by overproduction of synthesized polyglucose particles, especially amorphous particles with a diameter of less than 100 Å. The enzymatic histochemical reaction is apt to appear more intense in vitro than in vivo. Compared with polyglucose particles synthesized in vitro by glycogen synthetase or phosphorylase, polyglucose particles histochemically synthesized in vivo showed a close resemblance to native glycogen particles. Although there is no difference between the fundamental findings of the in vivo and in vitro experiments in such areas as localization and distribution of enzyme activity, the in vivo histochemistry can give more accurate information about the living cell.
日本組織細胞化学会の論文