ERp29 is a Radiation-Responsive Gene in IEC-6 Cell
スポンサーリンク
概要
- 論文の詳細を見る
ERp29 is a resident protein of the endoplasmic reticulum (ER) lumen, which is thought to be involved in the folding of secretory proteins. In our previous work, it was found that, when treated with ionizing radiation (IR), the ERp29 expression was increased in mouse intestinal epithelia and cultured IEC-6 cells, which suggested that ERp29 might be a radiation-induced gene. The current work is to confirm the induction of ERp29 by IR and to analyze its role in irradiated IEC-6 cells. Our results showed that ERp29 expression was elevated by IR in IEC-6 cells at mRNA and protein levels in a time-dependent manner. IEC-6 cells with different exogenous ERp29 expression were obtained by transfection with sense and antisense expression vectors of ERp29 coding region. As ERp29 expression was inhibited, these cells exhibited more serious radiation injury and more sensitivity to IR-induced apoptosis. To further elucidate the induction of ERp29, we analyzed the XBP1 expression after IR. Results showed that the spliced form of XBP1 mRNA rapidly reached a peak at 3 hours after irradiation, which indicated that UPR sensor was involved in radiation and might be a reason to induce ERp29 expression. Our results demonstrate that ERp29 is a radiation associated protein and plays an important role in protecting cells from IR.
- 日本放射線影響学会の論文
- 2008-11-16
著者
-
Wang Yan
Department Of Electrical Engineering National Cheng Kung University
-
Wang Yan
Department Of Medical Genetics Third Military Medical University
-
SU Yongping
Institute of Combined Injury, Third Military Medical University
-
AI Guoping
Institute of Combined Injury, Third Military Medical University
-
WANG Junping
Institute of Combined Injury, Third Military Medical University
-
WANG Meng
Institute of Modern Physics, CAS
-
ZHANG Bo
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Milit
-
YANG Yuan
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Milit
-
PANG Xueli
Department of Oncology, Southwest Hospital, Third Military Medical University
-
ZOU Zhongmin
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Milit
-
Pang Xueli
Department Of Oncology Southwest Hospital Third Military Medical University
-
Zou Zhongmin
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury Third Military
-
Wang Yan
Department Of Cardiovascular Sciences Oita University School Of Medicine
-
Zhamg Bo
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury Third Military
-
Wang Meng
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury Third Military
-
Ai Guoping
State Key Laboratory Of Trauma Burns And Combined Injury Institute Of Combined Injury College Of Pre
-
Su Yongping
State Key Laboratory Of Trauma Burns And Combined Injury Institute Of Combined Injury College Of Pre
-
Wang Junping
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury Third Military
-
Yang Yuan
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury Third Military
-
Wang Yan
Department Of Biology Graduate University Of Chinese Academy Of Sciences
関連論文
- Morphological relationship between nerve fibers and Langerhans cells in the epidermis of psoriasis vulgaris and lichen simplex chronicus
- Characterization of the Isoflavone Pratensein as a Novel Transcriptional Up-Regulator of Scavenger Receptor Class B Type I in HepG2 Cells
- In Vitro and in Vivo Antifungal Activities of the Eight Steroid Saponins from Tribulus terrestris L. with Potent Activity against Fluconazole-Resistant Fungal(Pharmacology)
- OE-135 Transcription Factors Csx/Nkx2.5 and GATA4 Distinctly Regulate Expression of Ca^ Channels in Neonatal Rat Heart(Development and differentiation-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- EXPERIMENTAL STUDY FOR THE TOXICOLOGICAL EFFECTS OF SVATE-3 ON RAT LIVER : OBSERVATION BY LIGHT AND ELECTRON MICROSCOPE AND BY ENZYME HISTOCYTOCHEMISTRY
- A study Assessing the Genotoxicity in Rats after Chronic Oral Exposure to a Low Dose of Depleted Uranium
- SL-1-1 The intestinal injuries by radiation, and the related work in China(Radiation Biology Research in Asia, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Effects of Shikonin Isolated from Zicao on Lupus Nephritis in NZB/W F1 Mice
- PJ-198 Ethanol-induced Tachycardia is Mediated by T-type Ca^ Channel Regulation Caused by Transcription Factor Csx/Nkx2.5 Activation(Arrhythmia, basic(05)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Socie
- Possible Proton Halo and Skin in Light Proton-Rich Nucleus
- ERp29 is a Radiation-Responsive Gene in IEC-6 Cell
- Involvement of Peroxiredoxin I in Protecting Cells from Radiation-Induced Death
- Structure-Activity Relationship of Polyphenols That Inhibit Fatty Acid Synthase
- 22pGS-2 Ambipolar injection of carriers and its application to light emission in organic field effect transistors
- High-Frequency Genetic Contents Variations in Clinical Candida albicans Isolates
- Roles of Different Areas of Palatine Bone Denudation on Growth and Development of the Maxilla and Dental Arch : An Experimental Study
- Device Linear Improvement Using SiGe/Si Heterostructure Delta-Doped-Channel Field-Effect Transistors
- The role of contrast-enhanced ultrasound on the diagnosis of small hepatocellular carcinoma (≦3cm) in patients with cirrhosis
- Isolation and Identification of Mycobacteria from Soils at an Illegal Dumping Site and Landfills in Japan
- Scoliosis Model Created by Pedicle Screw Tethering in Immature Goats : The Feasibility, Reliability, and Complications
- Improved capillary electrophoresis conditions for the separation of kinase substrates by the laser micropipet system
- Triterpenoid Saponins from the Seeds of Celosia argentea and Their Anti-inflammatory and Antitumor Activities
- A New Silicon Field-Effect Transistors with Two-Hole-Transport-Mode (HTM) Channels Grown by Molecular Beam Epitaxy (MBE)
- Inhibition of ErbB2 by Herceptin reduces survivin expression via the ErbB2-β-catenin/TCF4-survivin pathway in ErbB2-overexpressed breast cancer cells
- Effect of Pore Former on the Properties of Casted Film Prepared from Blends of Eudragit^[○!R] NE 30D and Eudragit^[○!R] L30 D-55
- Uterotrophic effects of cow milk in immature ovariectomized Sprague-Dawley rats
- Nonapeptide Hormones Oxytocin and Vasopressin Distinctly Regulate Ca_v1.2 L-type Calcium Channel Expression in Cardiomyocytes
- Protein identification assisted by the prediction of retention time in liquid chromatography/tandem mass spectrometry
- Prefrontal cortical cells projecting to the supplementary eye field and presupplementary motor area in the monkey
- Upregulation of δ-catenin is associated with poor prognosis and enhances transcriptional activity through Kaiso in non-small-cell lung cancer
- Classification of tumor size from view point of functional preservation
- Dynamical Structure of Water in Aqueous Solutions of Ascorbic Acid by Low-Frequency Raman Scattering
- Effect of cyclic hydrodynamic pressure-induced proliferation of human bladder smooth muscle through Ras-related C3 botulinum toxin substrate 1, mitogen-activated protein kinase kinase 1/2 and extracellular regulated protein kinases 1/2
- The Reproductive Effects in Rats after Chronic Oral Exposure to Low-dose Depleted Uranium
- Prediction of significant fibrosis and cirrhosis in hepatitis B e-antigen negative patients with chronic hepatitis B using routine parameters