NITROANILINEによるビタミンCの比色定量法 : (I)NITROANILNEとアスコルビン酸との反応および比色定量法への応用

概要

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In order to confirm the mechanism of the reaction of ascorbic acid with benzene diazonium salts, aniline and 13 kinds of its derivatives were examined. It was assumed that the presence of nitro radical in ortho or para position of aniline would be essential for the production of an intensely colored reaction medium, and also that methoxy group in the residual para or ortho position would serve as auxochrome group, while hydroxy and amino groups would not. It was found that p-nitroaniline gave a higher color reaction with a small amount of ascorbic acid (0.5-0.05mg) than that value with 4-methoxy-2-nitroaniline, and a linear relationship between the color intensity and the amount of ascorbic acid could be ascertained within the above meationed range of ascorbic acid, while this method and also 4-methoxy-2-nitroaniline method were interfered by the presence of glutathione, cysteine, glucose, xylose, and tannine in the test solutions.
日本ビタミン学会の論文