Spirulina platensisのMixotrophic Cultureにおけるアミノ酸の影響

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In the mixotrophic culture of Spirulina platensis, the effect of amino acids on the growth are summarized as follows : 1. S. platensis utilized alanine, arginine, aspartic acid, asparagine, cystine, glutamic acid, isoleucine, leucine, methionine, ornithine, proline, serine, tryptophan, and tryrosine. Among them, the most rapid cellular growth was observed with asparagine. glutamine, glycine, histidine, lysine, phenylalanine, threonine and valine were not utilized.2. The growth on asparagine medium was inhibited remarkably by the addition of phenylalanine, glutamic acid, or glutamine.3. Growth inhibition by the addition of phenylalanine in asparagine medium was observed at 10^<-3>M concentration and was recovered by the addition of oxaloacetate, pyruvate, or valine. Experiments suggested that phenylalanine blocks the metabolic step from aspartic acid to oxaloacetic acid, but the activity of glutamate oxaloacetate transaminase, which was extracted from S. platensis as the cell free preparation, was not inhibited by phenylalanine.4. Phenylalanine inhibited the utilization of arginine, cystine, glutamic acid, isoleucine, leucine, methionine, ornithine, proline and serine, also.5. The growth inhibition by glutamic acid in asparagine medium was recovered by the addition of oxaloacetic acid or α-ketoglutaric acid.6. It was presumed that the growth inhibition by glutamine medium depended on the concentration of ammonium which was released from glutamine.
社団法人日本生物工学会の論文
1973-05-25

Spirulina platensisのMixotrophic Cultureにおけるアミノ酸の影響

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