L-Dopa中間体, 3-(3,4-Dimethoxyphenyl)-L-alanine および3-(3,4-Methylenedioxyphenyl)-L-alanineの生産 : 醗酵法によるアミノ酸の工業的製造報に関する研究(第7報)

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The fermentative production of 3,4-disubstituted-phenyl-L-alanine, L-Dopa intermediates, was studied.1) 3-(3,4-Dimethoxyphenyl)-L-alanine and 3-(3,4-methylenedioxyphenyl)-L-alanine were formed from the corresponding α-hydroxy-, keto-, thioketo- or bromo-carboxylic acids by resting cells or fermentation using bacteria, molds, Streptomyces or yeasts.2) Enzymatic reaction conditions for the formation of 3-(3,4-dimethoxyphenyl)-L-alanine from 3,4-dimethoxyphenylpyruvic acid by resting cells of Brevibacterium roseum ATCC 13825 were studied. Wet, freeze-dried or vacuum-dried cells could be used and a pH of 7〜8 and a temperature of 30℃ were adequate. Glutamic acid and aspartic acid were good amino-group donors. The reaction was carried out in a 5l jar fermenter, ad 8 g/l of the L-amino acids was formed.
社団法人日本生物工学会の論文
1975-07-25