DL-α-ヒドロキシ酪酸またはDL-α-ブロム酪酸を原料とするイソロイシン生産菌のスクリーニング : 醗酵法によるアミノ酸の工業的製造法に関する研究(第1報)

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Screening of L-isoleucine-producing bacteria was carried out on media containing DL-α-hydroxybutyric acid (HB) and DL-α-bromobutyric acid (BB) as precursors. Besides Bacillus and Pseudomonas, which are already known as isoleucine-producers, the following bacteria have also been found to produce L-isoleucine from HB : Aerobacter, Brevibacterium, Corynebacterium, Escherichia, Flavobacterium, Micrococcus, Sarcina, and many others. Among the above, Brevibacterium ammoniagenes IAM 1641 was cultured in shaking flasks containing 2% HB, 10% cane molasses (as glucose), 0.9% urea, 1% corn steep liquor, and 0.5% proflo at 30℃ for 3 to 4 days, and 7 to 8 g L-isoleucine was produced in 1l medium. The same production rate was also obtained in 5l jar fermenters. BB as well as HB was found to be used as a precursor in the production of L-isoleucine and BB showed almost the same production rate as HB. Using BB as a precursor, several strains of L-glutamic acid producers were cultured and all of those were found to accumulate L-isoleucine at a high concentration. For instance, Brevibacterium roseum ATCC 13825 produced 12.3 g L-isoleucine in 11 medium containing 3.2% BB.
社団法人日本生物工学会の論文
1973-07-25