アミノ酸醗酵液よりのアミノ酸回収と菌体分離 : 醗酵法によるアミノ酸の工業的製造法に関する研究(第4報)

概要

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Isolation of amino acid and the separation of bacterial cells from amino acid fermented broth was studied.Heat treatment of culture broth at pH 4 was effective in decreasing filtration resistance, and the addition of a flocculant with the same heat treatment was more effective Celite 545 was most effective amoung the diatomaceus earths tested. A large amount of filter aid was needed for filtration of culture broth by filter press, oliver filter or basket centrifuge.Isoleucine could be isolated from the cultured broth without separation of bacterial cells, when the cultured broth was passed through an ion exchange resin column using the upward flow method.Sedimentation rate of bacterial cells was accelerated by the addition of flocculant with heat treatment at pH 4,and also by high temperature, at pH 4. Bacterial cells could be isolated using tubular-bowl or disk-bowl nozzle centrifuge. The disk-bowl nozzle centrifuge is suitable for commerical scale separation.
社団法人日本生物工学会の論文
1974-07-25