イソロイシン醗酵菌体びL-アスパラギン酸生産への再利用 : 醗酵法によるアミノ酸の工業的製造法に関する研究 (第5報)

概要

論文の詳細を見る
Strong aspartase acitivity was found in waste cells (Brevibacterium reseum ATCC 13825) of isoleucinew fermentation, and the production of L-aspartic acid from fumaric acid by re-use of the cells was studied.1) A liter of 10 per cent fumaric acid solution (adjust pH to 7.5 with NH_4OH)_3 120 g of wet cells (weight loss on drying was 80 per cent) and 1 g of cation surfactant were incubated at 45℃ for 48 hr, and aspartic acid was formed in a yield (mole) of more than 80 per cent.2) No difference in aspartace activity was observed between cells grown in Medium C1(cane molasses was used as the carbon source) and Medium G2 (glucose was used as the carbon source).3) L-Aspartic acid crystals, which passed the Japanese industrial standards for guaranteed ragents, were obtained from the supernatant of the reached fluid by isolelectric precipitation in a yield of 86 per cent.4) Sodium aspartate crystals, which passed the Japanese standards for food additives, were obtained from aspartic acid in a yield of 97 percent.5) Isoleucine fermentation was carried out in a 100 l fermenter, and 16.3 g/l of isoleucine was formed. Seven hundred and sixty six g of waste cells (dry weight) were obtained from the 45 l cultured broth, and 3,070 g of L-aspartic acid was formed from fumaric acid by the aspartase of the cells in a yield (mole) of 83.7 per cent.
社団法人日本生物工学会の論文
1974-07-25