DL-α-ブロム酪酸からイソロイシン生産における培地条件の検討 : 醗酵法によるアミノ酸の工業的製造法に関する研究(第2報)

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Culture media for the fermentative production of L-isoleucine from DL-α-bromobutyric acid (BB) were studied.1) The purity of BB was determined through titration of acidity and amount of Br^- released by hydrolysis.2) Almost all of the BB in a 16% solution (neutralized to pH 7.0 with ammonia water) was hydrolyzed when heated at 100℃ for 30min.3) No difference was observed between BB and DL-α-hydroxybutyric acid (HB) as precursors for cell growth and isoleucine formation.4) Butyric acid was contained in BB as an impurity. One per cent of butyric aicd in a medium inhibited both the growth of Brevibacterium roseum ATCC 13825 and the formation of isoleucine. But in the case of two other strains (Brevibacterium, Micrococcus), no inhibition was observed.5) Isoleucine was formed in high yields from a medium containing 3.2% BB. But, high concentrations of BB (4.8%, 6.4%) in a medium inhibited cell growth and isoleucine formation.6) The proper concentration of urea was 0.8%. Urea concentration of less than 0.8% resulted in the decrease of pH and a low yield, even if the pH of the medium was controlled.7) When certain organic nutrients, corn steep liquor and Proflo, were omitted, both cell growth and isoleucine formation were delayed when a 1% inoculum was used, but not when a 5% inoculum was used.8) As a result of the above studies, Brevibacterium roseum ATCC 13825 was cultured in a medium containing 10% cane molasses, 3.2% BB, 1% corn steep liquor, 0.5% Proflo, and 0.8% urea at 30℃ for 4days, and 12-14g/l of isoleucine was accumulated.9) A medium containing sucrose as the carbon source was studied and 16.7g/l of isoleucine was accumulated in the following medium : 10% sucrose, 3.2% BB, 1% corn steep liquor, 0.5% Proflo, 0.9% urea, 0.1% K_2HPO_4,0.05% MgSO_4・7H_2O, 30μg/l biotin, and 500μg/l thiamine.10) A medium containing glucose as the carbon source was studied and 15.2g/l of isoleucine was accumulated in the following medium : 10% glucose, 3.2% BB, 1% corn steep liquor, 0.5% Proflo, 0.9% urea, 0.1% CaCl_2・2H_2O, 0.05% MgSO_4・7H_2O, 30μg/l biotin, and 500μg/l thiamine
社団法人日本生物工学会の論文
1973-11-25

DL-α-ブロム酪酸からイソロイシン生産における培地条件の検討 : 醗酵法によるアミノ酸の工業的製造法に関する研究(第2報)

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