パン酵母培養廃液の処理及び利用に関する研究 : (第3報)Stysanus mediusの産生する抗かび性物質について

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In previous papers, treatment of baker's yeast culture waste solution was studied and it was found that soil fungi, Stysanus medius remove BOD in the waste solution effectively without dilution, and produce a antifungal antibiotic in mycelium simultaneously. This report concerns the new antifungal antibiotic. The results found were as follows. 1) The antibiotic was purified from extract of the mycelium with acetone. After removal of acetone, the remaining water layer was extracted with ethyl acetate. After removal of the ethyl acetate, the remaining oily substance was washed with ether and subjected to silica-gel column chromatography. A transparent crystal was obtained recrystallized from methanol and water. mp. 295°(decomp.)Elementary analysis and molecular weight determination indicated the molecula formula of C_<120>H_<221>N_<30>S_6O_<44>. Its ultraviolet and infrared absorption spectra and other physicochemical properties as well as its antimicrobial activity indicate it to be polypeptide, a new antibiotic, to which the name Stysadin was given.2) Amino acid composition of Stysadin are Thr 4,Ser 2,Glu 1,Pro 2,Gly 3,Ala 7,1/2 Cys 5,Val 3,Ileu 2,Ieu 4.3) Specifically, Stysadin inhibits partially growing of Hypochnus sasakii at 0.1〜0.2μg/ml and Penicillium digitatum at 0.2〜0.4 μg/ml.4) The tocity of Stysadin is low.
社団法人日本生物工学会の論文
1966-10-25