ビタミンB_<12>ならびにその関連化合物の化学的研究 : (XI)プロピオン酸菌の生産するニュクレオチド部を欠く補酵素型ビタミンB_<12>類縁体

概要

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A new yellow-orange substance was isolated from the cells of Propionibacterium shermanii grown in a medium containing cobinamide. The compound exhibited the coenzyme activity in the intramolecular oxidation-reduction system of Abeles・Lee. Its Km value was 6×10^<-6>M, 20 times as much as that of DBCC. It moved to cathode 1.5 times faster than cobinamide cyanide, hydroxo-cobalamin and DBCC at acidic pH in paperionophoresis, and gave the same spot as that of cobinamide cyanide after cyanide treatment. The shape of its absorption spectra in a neutral solution was analogous to that of DBCC except a difference in the wave length of the absorbancy maximum at 460mμ. The spectra was replaced by a spectra with double peaks at 350 and 500〜550mμ by exposure to light and changed to the spectra of cobinamide dicyanide by cyanide treatment. It exerted a little activity on E. coli mutant which was known to show a weak response to cobinamide and the derivatives. From these results, it would be concluded that the yellow orange substance was a coenzyme with cobinamide structure.
日本ビタミン学会の論文
1963-07-25