天然資源よりビタミンB_<12>結晶の製造に関する研究 : (XV)カラム分配クロマトグラフィーによるビタミンB_<12>の精製 : (3)活性汚泥抽出液活性炭処理後の粗製液への適用

概要

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Further investigations on column partition chromatographic purification of crude vitamin B_<12> solutions were pursued using aqueous solutions with lower purities than those used in the previous report, since it was presumed that the purification efficiency by the method would be dependent on the purity of the original solution. The solution used was prepared by eluting the vitamin from charcoal adsorbate with 60% aqueous acetone and by concentrating eluate under reduced pressure. Of three kinds of solvent systems examined, n-butanol saturated with phosphate buffer solution of pH 8 was found to be most efficient. In this case, the purity of the solution obtained by collecting appropriately the fractions from the column was 3.3%, and it corresponded to about 50 times of that of the original sample solution. This result is superior to our results reported previously. In addition, influence of amount as well as concentration of solution to be applied onto column upon the purification efficiency was studied.
日本ビタミン学会の論文