Eremothecium Ashbyii のビタミンB_2生産に關する研究 (第32報) : 培養物中のB_2の形態

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We studied on the forms of riboflavin produced by Eremothecium Ashbyii, using the paper chromatography technique.When cultivated in glucose-peptone basal medium, the most part of riboflavin in mycelium existed in free state, and flavin-adenine-dinucleotide was also found in trace. But the part which diffused into the culture solution contained no nucleotide-types.When the rice-embryo extract was added to the basal medium, an unidentified violet-fluorescent substance besides riboflavin and flacin-nucleotide was detected on the paper chromatogram. This substance was produced in rice-embryo culture, too.By fractionation with benzyl alcohol flavins in mycelium could be separated into nucleotide part and free diboflavin part.It was ascertained by the paper chromatography that the former was hydrolyzed to flavin-mononucleotide and free riboflavin and the latter was not changed when heated in 15% hydrochloric acid.
社団法人日本生物工学会の論文
1952-01-15