ビタミンB_<12>資源中のビタミンB_<12>類似体の検討 : (I)ビタミンB_<12>類似体の濾紙電気泳動法による分離

概要

論文の詳細を見る
The present paper deals with the fundamental investigations on the paper electrophoretic separation of a series of compounds of the vitamin B_<12> groups. In this research were used crystalline cyanocobalamin and almost pure preparations of-Factor A, Factor B, Factor III and pseudovitamin B_<12> that were kindly furnished by Dr. K. Bernhauer. Satisfactory separation of all these factors except Factor III was attained under the following conditions ; electrolyte : 0.5 or 1.0 N acetic acid containing 0.01% KCN, electrode vessel solution : 1% boric acid, electric current density : 0.1〜0.3 mA/cm, potential gradient : 10〜20 V/cm, paper : Toyo filter paper No. 50,time operated : more than 2 hours (but, more than 5 hours' migration is desirable in case of bioautographic detection.
日本ビタミン学会の論文