天然資源よりビタミンB_<12>結晶の製造に関する研究 : (XIV)カラム分配クロマトグラフィーによるビタミンB_<12>の精製 : (2)イオン交換クロマトグラフィー工程の代用としての試験

概要

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Investigations were made on the availability of column partition chromatographic procedure for one of the processes for the manufacture of crystalline vitamin B_<12> from activated sludge. Partially purified vitamin B_<12> solutions having purities of 0.5〜0.9% were used as sample solutions. By developing with solvent systems containing n-butanol as a main component and by collecting the appropriate fractions, vitamin B_<12> solutions of 7〜42% could be obtained. It is concluded from these results that the column partition chromatographic method could replace the ion exchange chromatographic procedure which had been adopted in the previous pilot plan. The evidence was also supplied suggesting the presence of a compound closely related to cyanocobalamin in the extract from activated sludge.
日本ビタミン学会の論文