天然資源よりビタミンB_<12>結晶の製造に関する研究 : (XIII)カラム分配クロマトグラフィーによるビタミンB_<12>の精製 : (1)純ビタミンB_<12>を用いる予備実験

概要

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Fundamental experiments on column partition chromatography for vitamin B_<12> were conducted with the view of selecting the solvent system and the innert supporter most suitable for applying the technique to one stage of processes for manufacturing crystalline vitamin B_<12> from activated sludge. In the first place, various solvent systems which contained n-butanol as a main component were examined, using powdered cellulose column. In case of water-saturated n-butanol, R value of the vitamin was about 0.24 and the degree of diffusion of zone was moderate. The addition of HCl, NaCl, KCN or phosphate to the system resulted in larger R value, but exerted little influence on the degree of diffusion. Also, organic compounds, such as methanol, iso-propanol and phenol, were used as an additional component in solvent systems and proved to promote the rate of movement of zone. Secondly, tests were made on the systems composed of sec-butanol and water in different proportions. Water-saturated sec-butanol was unsuitable, because it caused a considerable diffused zone. However, more distinct zones were obtained when water added to sec-butanol was reduced. Finally, our comparison tests on several kinds of innert supporters showed that powdered cellulose was most suited.
日本ビタミン学会の論文