ビタミンB_<12>資源調査 : (VIII)アルコール蒸溜廃液消化槽脱離液より菌体画分採取の工業試験

概要

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In our previous papers, it was shown that the bacterial cell fraction obtained by centrifugation from supernatant liquor of methane fermentation digester of distillers' wastes contained high vitamin B_<12> activities and might be useful as an industrial raw material for the preparation of crystalline vitamin B_<12>. Therefore, in order to collect the new vitamin B_<12> resources, the Sharples type supercentrifuge was operated 20 times at the Ishioka Distillers' Factory in Ibaraki Prefecture. About 16 kg of the dried cell fraction (13.5% moisture) was obtained, corresponding to 0.4% of the supernatant liquor treated. The vitamin B_<12> content of the mixed sample of bacterial cell fraction, estimated with E. coli, L. leichmannii and Ochromonas malhamensis, was 50,43 and 30 μg/g on the dry basis, respectively. The supernatant liquor of distillers' wastes digester seems therefore to be superior to that of night soil digester in view point of high yield and high vitamin B_<12> content.