酵素試験紙法の1つの応用 : アリルスルファターゼ生成に関してのタイプの異った細菌の分離

概要

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Fourteen strains of bacteria were isolated from soild, to be able to grow on either ρ-carboxyphenyl sulphate, tyrosine-o-sulphate or phenyl sulphate as the sole sulphur source. These strains, 2 strains of Aerobacter aerogenes and 2 strains of Pseudomonas aeruginosa were examined for the ability of arylsulphatase formation in culture medium containing inorganic sulphate with and without tyramine, taurine or ρ-carboxyphenyl sulphate as sulphur source. The test paper method showed that inorganic sulphate decreased the rate of arylsulphatase formation of all of the arylsulphatase positive strains tested. There were two types of bacteria in thye arylsulphatase formation, one being affected by tyramine and the other being little or not at all affected. Almost all of these results agreed with those of the experiments by estimation of arylsulphatase activities of the cells harvested from the various culture media. Therefore, there may be a possibility that the tesr paper method affords a new technique to isolate different types of bacteria concerned with the formation of some hydrolases as well as will arylsulphatase.
社団法人日本生物工学会の論文
1963-03-15