Phytophthora capsiciの遊走子の運動に関する研究 : II. 照度計による遊走子集泳状態のとらえ方

概要

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A new photometrical device for measuring the tactic aggregation of zoospores of Phytophthora capsici Leon. is described in this paper. An annular diaphragm (×l00) of Nikon phase contrast microscope was employed for dark ground illumination and reflected light intensity in the microscopic field was measured by an ordinary exposure meter for microscopic photography. Specimen chamber for the microscopic observation was devised by combining glass slide with cover glasses. When tips of plant roots, platinum electrodes, or capillary tubes containing plant extracts were introduced into the zoospore suspension in the specimen chamber, Various patterns of tactic aggregation of zoospores were observed. The light reflected by zoospores in the dark ground illumination was photometrically read and recorded. The photometrically plotted diagrams directly reflected the density of zoospore population in the specimen chamber. This method would certainly be applicable to the kinetic analysis of the tactic aggregation of zoospores.(Received May 2l, 1966)
日本植物病理学会の論文
1967-01-30