Purification and Identification of the Promoter of Sediment Formation from Raw Soy Sauce by Heating

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概要

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• Soy sauce contains promoters of sediment formation at 60℃. These promoters were extracted with 1-butanol from raw soy sauce, followed by acetonitrile extraction. The acetonitrile-soluble fraction was used for further separation of the promoter of sediment formation, as the specific activity of the acetonitrile-soluble fraction was higher than that of the insoluble fraction. The promoter of sediment formation could be separated as a single peak from the main low-molecular weight peak on a Sephadex G-10 column by means of HPLC on a LiChrospher(RP-18)column. The active fraction for sediment forming-activity separated by three columns, namely, a LiChrospher(RP-18)column, an Alltech 700CH carbohydrate column and a Shodex OHpak Q-801 column, was observed as a single peak. 0.08 g of promoter was obtained from 1 l of raw soy sauce. The ^<13>C, ^1H NMR and ^1H-^1H COSY spectral data identified the purified promoter as _L-glutamic acid 5-n-butyl ester. _L-Glutamic acid 5-n-butyl ester was synthesized and identified as a promoter by HPLC and ^1H NMR analysis. In addition, the existence of _L-glutamic acid 5-n-butyl ester in raw soy sauce was confirmed by HPLC analysis of the extract without 1-butanol extraction from raw soy sauce.

• 社団法人日本生物工学会の論文
• 1998-10-25

著者

• KITAHARA Haruo

  Department of Natural Science, Faculty of Education, Hirosaki University

• YOSHIKI Yumiko

  Department of Environmental Bioremediation, Tohoku University

• OKUBO Kazuyoshi

  Department of Food Chemistry, Faculty of Agriculture, Tohoku University

• MOTOMURA Yoshie

  Laboratory of Science of Bioproducts, Faculty of Agriculture and Life Science, Hirosaki University

• TOMITA Masahiro

  Department of Chemistry for Matrials, Mie University

• Yoshiki Y

  Tohoku Univ. Miyagi Jpn

• Yoshiki Yumiko

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Kitahara Haruo

  Department Of Natural Science Faculty Of Education Hirosaki University

• Okubo K

  Tohoku Univ. Miyagi Jpn

• Okubo K

  Department Of Food Chemistry Facultv Of Agriculture Tohoku University

• Okubo Kazuyoshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Motomura Yoshie

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Tomita M

  Tohoku Women's Junior College

• Tomita Masahiro

  Department Of Chemistry For Materials Faculty Of Engineering Mie University

• Tomita Masahiro

  Department Of Biology. Tokyo Metropolitan University

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