A Radioimmunoassay of Plasma 18-Hydroxy-11-Deoxycorticosterone
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概要
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A radioimmunoassay for the measurement of plasma 18-hydroxy-11-deoxycorticosterone (18-OH-DOC) has been developed. The antiserum for 18-OH-DOC was produced in rabbits immunized with 18-OH-DOC-3-(0-carboxymethyl) oxime-bovine serum albumin. This antiserum was used at a final dilution of 1: 40, 000 and the main cross reactants were aldosterone at 29.4%, 18-hydroxy-corticosterone at 4.9% and 11-deoxycorticosterone at 3.6%. Silica gel column chromatography (7×30mm, toluene saturated with propylene glycol: dichloromethane, 95: 5) and Sephadex LH-20 column chromatography (7×60mm, benzene: methanol, 85: 15) were employed for the separation of these cross reactants. The sensitivity was 10pg and the method blank was consistently negligible. The intra- and inter-assay precision was 11.3% and 14.8%, respectively.
- 社団法人 日本内分泌学会の論文
著者
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KAMBEGAWA AKIRA
Department of Obstetrics and Gynecology, Faculty of Medicine, Teikyo University
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Takagi Shigeo
Department Of Electrical Engineering Faculty Of Engneering Shizuoka University
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YOSHIDA Takao
Department of Gynecology Hoshi General Hospital
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HONDA MASANOBU
The Second Department of Internal Medicine, Nihon University School of Medicine
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Honda Masanobu
The Second Department Of Internal Medicine Nihon University School Of Medicine Tokyo Japan
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Den Konbai
Department of OBGYN Nihon UNivercity School of Medicine
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Tomita Masahiro
Department Of Biology. Tokyo Metropolitan University
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TOCHIGI BUICHI
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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Yoshida Takao
Department Of Biology Faculty Of Science Hokkaido University
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TOMITA MASAHIRO
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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KAMBEGAWA AKIRA
Department of Chemistry, Research Laboratories, Teikoku Hormone Mfg. Co.
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