Study of Oxytocin Receptor in Human Myometrium Using Highly Specific <SUP>3</SUP>H-Labeled Oxytocin
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概要
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A highly specific tritium labeled oxytocin (<SUP>3</SUP>H-OT) was synthesized utilizing the method of catalytic substitution of halogen for hydrogen. The specific activity of 3H-OT was19Ci/mM and the biologic activity was 350U/mg, which was sufficient for the OT radioreceptor assay. The maximum % uptake of <SUP>3</SUP>H-OT in the human myometrium was observed in 20, 000×<I>g</I> pellets under the optimal conditions of pH7.4, at20°C and the incubation time of 90min and it was augmented in the presence of Mn<SUP>++</SUP>. It was also observed from the Scatchard plot, that the binding site of OT in the human myometrial specimens was a single type within the range of OT concentration of 0.4nM to 1.6nM. The dissociation constants (Kd) and the number of binding sites (NBS) showed a relative increase as gestation advance. The apparent Kd of term pregnancies was 1.25×10<SUP>-9</SUP>M regardless of the presence or absence of labor pains, while the NBS of term pregnancies with and without labor pain was 1.2×10<SUP>-12</SUP> and 4.7×10<SUP>-12</SUP>moles/mg protein, respectively.
- 社団法人 日本内分泌学会の論文
著者
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YAMAMOTO Koichi
Department of Geriatric Medicine, Osaka University Graduate School of Medicine
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Takagi Shigeo
Department Of Electrical Engineering Faculty Of Engneering Shizuoka University
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YOSHIDA Takao
Department of Gynecology Hoshi General Hospital
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OYAMA Yoichi
Departments of Radiology, Kumamoto University School of Medicine
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Den Konbai
Department of OBGYN Nihon UNivercity School of Medicine
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Sakamoto Hideki
Department Of Obgyn Nihon Univercity School Of Medicine
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Sakamoto Hideki
Department Of Gynecology Tokyo Medical And Surgical Clinic
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ARAI TAKENORI
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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KAWAI SHUN
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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Yamamoto Koichi
Department Of Electrical Engineering College Of Engineering University Of Osaka Prefecture
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Yoshida Takao
Department Of Biology Faculty Of Science Hokkaido University
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OYAMA YOICHI
Department of Obstetrics and Gynecology, Nihon University School of Medicine
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SAKAMOTO Hideki
Departmemt of Obstetrics and Gynecology,Nihon University School of Medicine
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