LOCALIZATION OF IMMUNOREACTIVE STEROIDS IN HUMAN CHORIONIC VILLI
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概要
- 論文の詳細を見る
The authors investigated the localization of progesterone (P) and estradiol (E_2) in human chorionic villi by immunohistochemical techniques and obtained the results to be presented in this report. 1. The tissues were fixed in 1.25-2.5% glutaraldehyde (GA) including 10% glycerol at 4℃ for 2-4 hr, then washed in phosphate buffer including 10% glycerol, frozen and microtomed to produce adequate specimens for further processing and evaluation. 2. The immunostaining was done with either anti P or anti E_2 antisera, which were adsorbed with either bovine serum albumin (BSA) or steroid extracted powdered placentas to eliminate non-specific reactions. 3. The cytoplasm of the syncytiotrophoblastic layer and Langhans cells of chorionic villi from the first and second trimester, and the cytoplasm of the syncytiotrophoblastic layer excluding the areas of the vasculosyncytial membranes of chorionic villi in late pregnancy revealed the presence of immunoreactive P and E_2.
- 日本組織細胞化学会の論文
著者
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TAKAGI Shigeo
Department of Mechanical Engineering, Nihon Institute of Technology
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KAMBEGAWA AKIRA
Department of Obstetrics and Gynecology, Faculty of Medicine, Teikyo University
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Takagi Shigeo
Department Of Obs. And Gyne. Nihon University School Of Medicine
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Takagi Shigeo
Department Of Electrical Engineering Faculty Of Engneering Shizuoka University
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Kanbegawa Akira
Teikoku Hormone Mfg. Co. Ltd.
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NAKAMURA HARUYUKI
Department of Gynecology, Nihon University School of Medicine
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TSUBATA KATSUO
Department of Gynecology, Nihon University School of Medicine
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Nakamura Haruyuki
Department Of Obs. And Gyne. Nihon University School Of Medicine
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Tsubata Katsuo
Department Of Obs. And Gyne. Nihon University School Of Medicine
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KAMBEGAWA AKIRA
Department of Chemistry, Research Laboratories, Teikoku Hormone Mfg. Co.
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