Effect of Hydrocarbon-Water Interfaces on Synthetic and Hydrolytic Activities of Lipases
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概要
- 論文の詳細を見る
We have developed a new method of lipase activation for interesterification, in which lipase, in contact with a hydrocarbon-water interface, has been found to have high interesterification activity in an anhydrous solvent. We have applied this activation method to various lipase and obtained high synthetic activity in n-hexane, and have investigated the effect of various hydrocarbon-water interfaces on the synthetic and hydrolytic activities of lipases. The esterification and/or interesterification activity of lipases tested was improved by this activation method, using an n-tetradecane-water interface. From the initial group of lipases, three representative lipases(from Rhizopus japonicus, Chromobacterium viscosum and porcine pancreas)were selected for further study. The effect of various hydrocarbon-water interfaces on synthetic(interesterification or esterification)activity was studied. We demonstrated that the resulting synthetic activity was affected by the choice of hydrocarbon-water interface and that there were differneces in the effects of interfaces on the synthetic activity of these lipases.
- 社団法人日本生物工学会の論文
- 2001-09-25
著者
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丸山 達生
神戸大・工・応化
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NAKAJIMA Mitsutoshi
National Food Research Institute
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SEKI Minoru
Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University
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Nakajima Mitsutoshi
Food Engineering Division National Food Research Institute Naro
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Seki M
Univ. Tokyo Tokyo Jpn
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MARUYAMA Tatsuo
National Food Research Institute
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Seki Minoru
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
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Nakajima M
Food Engineering Division National Food Research Institute Naro
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