Anthocyanin Synthesis, Growth and Nutrient Uptake in Suspension Cultures of Strawberry Cells
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概要
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Strawberry(Fragaria ananassa cv.Shikinari)cell suspension cultures carried out in shake flasks for 18 d were closely examined for cell growth, anthocyanin synthesis and the development of pigmented cells in relation to the uptake of carbohydrate, extracellular PO_4, NO_3, NH_4, and calcium. Cell viability, extracellular anthocyanin content, pH and electrical conductivity of the broth were also monitored. The specific growth rate of strawberry cells at exponential phase was 0.27 and 0.28 d^<-1> based on fresh and dry weight, respectively. Anthocyanin synthesis was observed to increase continuously to a maximum value of 0.86 mg/g fresh cell weight(FCW)at day 6, and was partially growth-associated. Anthocyanin synthesis was linearly related to the increase in pigmented cell ratio, which increased with time and reached a maximum value of ca. 70% at day 6 due to reduction in cell viability and depletion of substrate. Total carbohydrate uptake was closely associated with increase in cell growth, and glucose was utilized in preference to fructose. Nitrate and ammonia were consumed until 9 d of culture, but phosphate was completely absorbed within 4 d. Calcium was assimilated throughout the growth cycle. After 9 d, cell lysis was observed which resulted in the leakage of intracellular substances and a concomitant pH rise. Anthocyanin was never detected in the broth although the broth became darkly pigmented during the lysis period. This suggests that anthocyanin was synthesized only by viable pigmented cells, and degraded rapidly upon cell death and lysis. Based on the results of kinetic analysis, a model was developed by incorporating governing equations for the ratio of pigmented cells into a Bailey and Nicholson's model. This was verified by comparison with the experimental data. The results suggest that the model satisfactorily describes the strawberry cell culture process, and may thus be used for process optimization.
- 公益社団法人日本生物工学会の論文
- 1998-07-25
著者
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Furusaki Shintaro
Department Of Applied Life Science Faculty Of Engineering Sojo University
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Furusaki Shintaro
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Zhang Wei
Department Of Cardiology Key Laboratory Of Cardiovascular Remodeling And Function Research Chinese M
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Seki Minoru
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
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Seki Minoru
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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MIDDELBERG ANTON
Department of Chemical Engineering, The University of Adelaide
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Middelberg Anton
Department Of Chemical Engineering The University Of Adelaide
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Zhang Wei
Department Of Anesthesiology First Affiliated Hospital Zhengzhou University
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Zhang Wei
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:department Of Biochemical Engineering Dalian Institute Of Chemical Physics Chinese Academy Of Sciences:department Of Chemical Engineering The University Of Ad
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