Continuous Separation of Proteins by Isoelectric Focusing
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概要
- 論文の詳細を見る
Continuous separation of proteins is studied by using electrophoresis. The apparatus is composed with electrophoresis cells which are separated by porous membranes. The value of pH is adjusted so as to be low near the anode and high near the cathode. Intermediate cells stand at intermediate values of pH. Some cells stand at pHs of the isoelectric points of desired proteins. By the driving forces originated by the electric potential field, proteins migrate toward the cells at their isoelectric points. This separation, continuous isoelectric focusing, is studied by measuring extent of migration of bovine serum albumin. Experimental results are discussed by use of a theory considering electrochemical mobility and flow pattern inside the cells.
- 日本膜学会の論文
著者
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Furusaki Shintaro
Department Of Applied Life Science Faculty Of Engineering Sojo University
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Kikuchi Tatsuji
Department of Chemical Engineering, University of Tokyo
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Furusaki Shintaro
Department of Chemical Engineering, University of Tokyo
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