Increased Alkaloid Production in a Suspension Culture of Coffea arabica Cells Using an Adsorption Column for Product Removal

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概要

• 論文の詳細を見る

• Since caffeine suppresses purine alkaloid (caffeine and theobromine) production by Coffea arabica cells, the effect of removing, caffeine from the medium was investigated using the hydrophobic resin Amberlite XAD-4. An in situ removal method did not result in increased production because the adsorbent removed not only the products, but also essential components (hormones). Therefore, the use of an adsorption column employing the hydrophobic resin was investigated with a 35-d semicontinuous culture under light irradiation. Caffeine was removed within 30 min by the adsorption column on day 23 of the cultivation. In this system, the negative effects of hormone removal by the adsorbent were canceled out by the addition of fresh medium. This method increased alkaloid production 1,4-fold compared to the culture with no removal treatment.

• 社団法人日本生物工学会の論文
• 1994-07-25

著者

• Kurata Hiroyuki

  Department of Bioscience and Bioinformatics, Kyushu Institute of Technology

• SEKI Minoru

  Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University

• Kurata H

  Univ. Tokyo Tokyo Jpn

• 古崎 新太郎

  崇城大学工学部応用生命科学科

• FURUSAKI Shintaro

  Department of Applied Life Science, Faculty of Engineering, Sojo University

• KAWAI ATSUSHI

  Department of Chemical Engineering, The University of Tokyo

• Kawai Atsushi

  Department Of Chemical Engineering The University Of Tokyo

• Kurata Hiroyuki

  Department Of Bioscience And Bioinformatics Kyushu Institute Of Technology

• Kawai Atsushi

  Department Of Astronomy Graduate School Of Science Kyoto University

• Furusaki Shintaro

  Department Of Applied Life Science Faculty Of Engineering Sojo University

• Seki Minoru

  Department Of Chemical Engineering The University Of Tokyo

• Furusaki S

  Univ. Tokyo Tokyo Jpn

• Seki Minoru

  Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University

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