Separation of Cultured Strawberry Cells Producing Anthocyanins in Aqueous Two-Phase System(BIOCHEMICAL ENGINEERING)
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概要
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A rapid and simple selection method of high-yield cells has been desired to establish highly productive cell lines for useful secondary metabolites. For this purpose, a new attempt was made to partition cultured plant cells in a poly(ethylene glycol)-dextran aqueous two-phase system (ATPS). The applicability of the ATPS in partitioning cultured strawberry cells (designated FAW) was investigated. The result of single-step partitioning in the ATPS supplemented with 0.4mmol/kg lithium sulfate showed that FAW cells cultivated for 7d under light-irradiation were separated into two cell populations with significantly different anthocyanin content. Additionally, the analysis technique of microscopic cell images showed that cells accumulating a high level of anthocyanin were partitioned completely into the bottom phase in a partitioning experiment of FAW cells cultivated for 10d under light-irradiation in the ATPS supplemented with 1.8mmol/kg potassium phosphate buffer. These results indicated that cell partitioning in ATPS increased the intracellular anthocyanin content and that the cultured strawberry cell population was heterogeneous in terms of cell surface properties. This is the first report of partitioning based on the heterogeneity of the cell surface properties correlated with the intracellular secondary metabolism in cultured plant cells. Our results also suggested that the ATPS was appropriate as a large-scale method for selecting useful cell lines among the cultured plant cells.
- 2005-10-25
著者
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NAKAMURA Masayuki
Department of Medical Simulation Engineering, Research Center for Nano Medical Engineering, Institut
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Miyanaga Kazuhiko
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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YAMADA Masumi
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo
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SEKI Minoru
Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University
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Kanamori Toshiyuki
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Edahiro Junichi
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo
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Seike Shinya
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo
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Kakigi Yasuhiro
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo
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Kanamori Toshiyuki
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Kanamori Toshiyuki
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Seki Minoru
Dep. Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba Univ. 1-33 Yayoi-ch
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Seike Shinya
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Yamada Masumi
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Kakigi Yasuhiro
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Edahiro Junichi
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:research Cen
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Seki Minoru
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
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Nakamura Masayuki
Department Of Chemistry Faculty And Graduate School Of Sciences Kyushu University
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Nakamura Masayuki
Department Of Animal Science Faculty Of Agriculture Tohoku University
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Miyanaga Kazuhiko
Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Tech
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Edahiro Jun-ichi
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:research Cen
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KANAMORI Toshiyuki
Research Center for Stem Cell Engineering (SCRC), National Institute of Advanced Industrial Science and Technology (AIST)
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